长双歧杆菌胞外多糖对非酶糖基化的抑制作用  

Inhibition of non-enzymatic glycosylation by exopolysaccharides of Bifidobacterium longum

作　　者：夏俊英 余海霞 戚仕梅 张富娜 邬婧 肖卫华 XIA Junying;YU Haixia;QI Shimei;ZHANG Funa;WU Jing;XIAO Weihua(School of Basic Medical Sciences,USTC Life Sciences and Medicine,University of Science and Technology of China,Hefei 230026,China;Institute of Advanced Technology,University of Science and Technology of China,Hefei 230031,China;Hefei Kadir Biotechnology Co.,Ltd.,Hefei 230000,China)

机构地区：[1]中国科学技术大学生命科学与医学部基础医学院,合肥230026 [2]中国科学技术大学先进技术研究院,合肥230031 [3]合肥卡迪尔生物科技有限公司,合肥230000

出　　处：《生物学杂志》2024年第5期6-13,47,共9页Journal of Biology

基　　金：中国科学技术大学先进技术研究院横向课题(JL05223001)。

摘　　要：利用人粪便中分离的一株长双歧杆菌ZJ1株胞外多糖提取物(ZJ1-EPS),分别采用丙酮醛(MGO)诱导牛血清白蛋白(BSA)和人皮肤细胞非酶糖基化(AGEs)模型和葡萄糖诱导斑马鱼AGEs模型,探究ZJ1-EPS抑制AGEs的作用和机制。结果表明,ZJ1-EPS对AGEs生成有明显的抑制作用,同时可显著抑制角质细胞和成纤维细胞中丙酮醛(MGO)诱导的MMP-1表达,促进胶原蛋白的表达。研究揭示了双歧杆菌胞外多糖提取物具有抑制AGEs生成的作用,提示其用于皮肤抗衰的作用机制和应用潜力。The extracellular polysaccharide extract(ZJ1-EPS)of Bifidobacterium longum ZJ1 strain isolated from the feces of centenarians was used to explore the effect and mechanism of ZJ1-EPS inhibition of AGEs by pyruvaldehyde(MGO)-induced bovine serum albumin(BSA)and non-enzymatic glycosylation(AGEs)in human skin cells and glucose-induced zebrafish AGEs,respectively.The results showed that ZJ1-EPS had a significant inhibitory effect on the formation of products in the middle and late stages of protein nonenzymatic glycosylation.This study revealed that the exopolysaccharide extract of Bifidobacterium has the effect of inhibiting the production of AGEs,suggesting its mechanism of action and application potential for skin anti-aging.

关 键 词：长双歧杆菌 胞外多糖 非酶糖基化 皮肤细胞 斑马鱼 

分 类 号：Q93[生物学—微生物学] R378[医药卫生—病原生物学]