功能化光子晶体表面增强拉曼光谱快速检测食品中黄曲霉毒素B1  

Rapid detection of aflatoxin B1 in food by surface-enhanced Raman spectroscopy of functionalized photonic crystals

作　　者：朱晨灵 霍冰洋 李攻科 Chenling Zhu;Bingyang Huo;Gongke Li(School of Chemistry,Sun Yat-sen University,Guangzhou 510006,China)

机构地区：[1]中山大学化学学院,广州510006

出　　处：《中国科学：化学》2024年第9期1607-1616,共10页SCIENTIA SINICA Chimica

基　　金：广东省自然科学基金(编号:2024A1515011077);国家自然科学基金(编号:22134007)资助项目。

摘　　要：本文采用垂直蒸发自组装法制备了功能化光子晶体表面增强拉曼基底(PC@AuNPs),建立了表面增强拉曼光谱(SERS)快速检测食品中黄曲霉毒素B1(AFB1)的分析方法.采用红外光谱、紫外吸收光谱、X射线光电子能谱和扫描电子显微镜表征了SERS基底.采用结晶紫为探针分子研究了SERS基底的活性,结晶紫和AFB1增强因子(EF)分别为2.0×10^(7)和1.2×10^(7),与电磁场增强模拟结果吻合,DFT理论预测AFB1拉曼光谱与实验测量光谱的特征峰吻合.选择1025.5 cm^(-1)作为AFB1拉曼定量峰,该方法的线性方程为I=5947.3lgc+24658,相关系数r为0.9933,线性范围为1.0~1.0×10^(4)μg L^(-1),检出限为0.46μg L^(-1).方法用于玉米、大豆和食醋样品中AFB1的检测,玉米和大豆的含量分别为7.40和15.1μg kg^(-1),加标回收率为95.8%~98.0%.新方法与高效液相色谱(HPLC)标准方法比较,分析结果的相对误差分别为-14%(玉米)和4.9%(大豆),功能化光子晶体SERS快速检测方法灵敏、准确和可靠,满足谷物样品中AFB1快速检测.In this study,a functionalized photonic crystal surface-enhanced Raman substrate(PC@AuNPs)was prepared using the vertical evaporation self-assembly method,and a surface-enhanced Raman spectroscopy(SERS)method for the rapid detection of aflatoxin B1(AFB1)in food was established.Fourier-transform infrared spectroscopy(FTIR),UV-Vis absorption spectroscopy,X-ray photoelectron spectroscopy(XPS),and scanning electron microscopy(SEM)were used to characterize the prepared substrates.The activity of the SERS substrate was studied using crystal violet as the probe molecule,with the enhancement factor(EF)for crystal violet and AFB1 being 2.0×10^(7) and 1.2×10^(7),respectively.These values were consistent with the simulated results of electromagnetic field enhancement.Additionally,the Raman spectra of AFB1 predicted by DFT theory had characteristic peaks that matched the experimental spectra.The Raman quantitative peak of AFB1 was selected at 1025.5 cm^(-1),and the linear equation of the method was I=5947.3lgc+24658,with a correlation coefficient of 0.9933,a linear range of 1.0~1.0×10^(4)μg L^(-1),and the limit of detection was 0.46μg L^(-1).This method was used to detect AFB1 in corn,soybean and vinegar samples,with detected levels of 7.40μg kg^(-1) in corn and 15.1μg kg^(-1) in soybean,and spiked recoveries ranging from 95.8% to 98.0%.The relative errors were -14% for corn and 4.9% for soybean when the new method was compared with the highperformance liquid chromatography(HPLC)standard method.The new SERS method is sensitive,accurate and reliable,meeting the requirements for the rapid detection of AFB1 in grain samples.

关 键 词：光子晶体 表面增强拉曼光谱 黄曲霉毒素B1 食品 

分 类 号：O657.37[理学—分析化学] TS207.5[理学—化学]