基于电子顺磁共振波谱分析高甾醇菜籽油的抗氧化性能  

Analysis of Antioxidant Properties of High-Sterol Rapeseed Oil by EPR Spectroscopy

作　　者：曹雄杰 吴卫国[1] 邱志鹏 夏炜杰 CAO Xiongjie;WU Weiguo;QIU Zhipeng;XIA Weijie(College of Food Science and Technology,Hunan Agricultural University,Changsha 410016;Changsha Institute for Food and Drug Control,Changsha 410016)

机构地区：[1]湖南农业大学食品科技学院,长沙410128 [2]长沙市食品药品检验所,长沙410016

出　　处：《食品工业》2024年第8期70-73,共4页The Food Industry

基　　金：湖南省市场监督管理局科技计划项目(2023KJJH30)。

摘　　要：自由基形成是菜籽油自氧化发生的原因,甾醇可以清除自由基,从而具有抗氧化性。自旋捕获剂对菜籽油中瞬态自由基捕获,采用电子顺磁共振波谱测定菜籽油体系中捕获自由基含量,得到低甾醇和高甾醇含量菜籽油在不同温度、不同储存容器、紫外线照射时间下氧化情况。在温度和贮藏容器影响下自由基强度和过氧化值无显著性差异(P<0.05)。高甾醇菜籽油抗氧化效果明显:7 d氧化后, 20, 40和60℃高甾醇菜籽油POV值分别比普通菜籽油减少了0.04, 0.14和0.34 g/100 g;金属、玻璃、塑料容器中高甾醇菜籽油POV值分别比普通菜籽油减少了0.20, 0.14和0.14 g/100 g;紫外灯能量破坏甾醇使其丧失抗氧化能力。The formation of free radicals is the reason for the self oxidation of rapeseed oil,and sterols can eliminate free radicals,thus possessing antioxidant properties.Spin trapping agents capture transient free radicals in rapeseed oil,and the content of captured free radicals in the rapeseed oil system is determined by electron paramagnetic resonance spectroscopy.The oxidation of rapeseed oil with low and high sterol contents is obtained at different temperatures,storage containers,and UV irradiation time.There is no significant difference in the intensity of free radicals and peroxidation values under the influence of temperature and storage containers(P<0.05).High sterol rapeseed oil has a significant antioxidant effect.After 7 days of oxidation,the POV values of high sterol rapeseed oil at 20,40 and 60℃decrease by 0.04,0.14 and 0.34 g/100 g,respectively,compare to ordinary rapeseed oil;The POV values of high sterol rapeseed oil in metal,glass,and plastic containers are reduced by 0.20,0.14 and 0.14 g/100 g,respectively,compare to ordinary rapeseed oil;The energy of ultraviolet lamps destroys sterols,causing them to lose their antioxidant capacity.

关 键 词：电子顺磁共振波谱 植物甾醇 菜籽油 抗氧化性 

分 类 号：O657.61[理学—分析化学] TS225.14[理学—化学]