贵州特有植物平坝槭叶绿体基因组特征分析  

Analysis on Chloroplast Genome Characteristics of the Unique Guizhou Species Acer shihweii

作　　者：周艳[1,2] 李婧 文香英 ZHOU Yan;LI Jing;WEN Xiangying(Guizhou Botanical Garden,Guiyang 550004,China;Key Laboratory for Biodiversity Conservation in Karst Mountain Area of Southwestern China of the National Forestry and Grassland Administration,Guiyang 550004,China;South China Botanical Garden,Chinese Academy of Sciences,Guangzhou 510650,China)

机构地区：[1]贵州省植物园,贵阳550004 [2]国家林业和草原局西南喀斯特山地生物多样性保护重点实验室,贵阳550004 [3]中国科学院华南植物园,广州510650

出　　处：《种子》2024年第9期1-7,15,共8页Seed

基　　金：国际植物园保护联盟项目(BGCI30432);国家自然科学基金委员会-贵州省人民政府喀斯特科学研究中心项目(U1812401)。

摘　　要：为研究贵州特有植物平坝槭叶绿体基因组结构与特征,以平坝槭新鲜叶片为材料,提取叶绿体DNA旨在对其基因组进行测序和组装,并进行相关分析。结果表明,平坝槭的叶绿体基因组长度为155069 bp,由大单拷贝区(LSC,84840 bp)、小单拷贝区(SSC,18093 bp)以及两个反向重复序列(IRA,26068 bp;IRB,26068 bp)组成。另外,还发现叶绿体基因组的GC含量为38%。经过注释,成功鉴定出87个基因,其中包括8个rRANs基因,37个tRNAs基因和42个蛋白编码基因。经系列分析,鉴定出的SSR位点有77个,散在重复系列有26个。正选择分析显示,rpl16、petD、ycf2、rpl22、rps16等5个基因出现正选择信号。密码子偏好性分析筛选到RSCU>1的密码子有32个,以T结尾的密码子最多,有16个。系统发育分析显示,平坝槭与亮叶槭亲缘关系较近。To investigate the structure and characteristics of the chloroplast genome in Acer shihwei,an unique species from Guizhou Province,chloroplast DNA was extracted from fresh leaves of Acer shihwei,followed by genome sequencing and assembly,of which correlation analysis was conducted.The results indicated that the chloroplast genome length of Acer shihwei was 155069 bp,consisted of a large single copy(LSC)region of 84840 bp,a small single copy(SSC)region of 18093 bp,and two inverted repeat regions(IRA,26068 bp and IRB,26068 bp).Furthermore,the GC content of the chloroplast genome was determined to be 38%.After annotation,a total of 87 genes were successfully identified,of which they included eight rRNA genes,thirtyseven tRNA genes,and fortytwo proteincoding genes.Through sequence analysis,seventyseven SSR loci were detected with twenty-six located within dispersed repeat sequences.Positive selection analysis revealed that the rpl16,petD,ycf2,rpl22,and rps16 genes exhibited signals indicative of positive selection.Codon usage bias analysis identified thirtytwo codons with RSCU values greater than one;notably those ending in T predominated with sixteen occurrences.Phylogenetic analysis demonstrated that Acer shihwei was closely related to Acer lucidum.

关 键 词：平坝槭 叶绿体基因组 密码子偏好性 系统发育分析 

分 类 号：S792.35[农业科学—林木遗传育种]