健康与患根腐病三七根际和根内微生物群落结构与多样性研究  

Microbial Community Structure and Diversity in Rhizosphere and Roots of Healthy and Root Rot Panax notoginseng

作　　者：丁子元 赵学强 李宁[4] 陈欣怡 孙玉婷 郑晓卫 李睿尧 夏冰 杨娟 DING Zi-yuan;ZHAO Xue-qiang;LI Ning(Nutrition&Health Research Institute,COFCO Corporation,Beijing 102209;Beijing Key Laboratory of Nutrition,Health and Food Safety,Beijing 102209;North China Pharmaceutical Group Aino Co.,Ltd.,Shijiazhuang,Hebei 052165;New Drug Research&Development Company of NCPC,Shijiazhuang,Hebei 050000)

机构地区：[1]中粮营养健康研究院有限公司,北京102209 [2]营养健康与食品安全北京市重点实验室,北京102209 [3]华北制药集团爱诺有限公司,河北石家庄052165 [4]华北制药集团新药研究开发有限责任公司,河北石家庄050000

出　　处：《安徽农业科学》2024年第19期151-157,共7页Journal of Anhui Agricultural Sciences

基　　金：河北省重大科技成果转化专项(20282901Z)。

摘　　要：[目的]研究三七健康植株和根腐病植株根内及根际土的微生物群落结构,探究其与三七根腐病发生的相关性。[方法]利用Illumina高通量测序技术对云南文山连作3年的健康植株根内及其根际土壤、根腐病植株根内及其根际土壤中的微生物群落结构及多样性进行分析。[结果]健康植株及其根际土壤与根腐病植株及其根际土壤中微生物群落的丰富度存在显著差异。黄腐植株根际土中的优势微生物为节杆菌属(Arthrobacter)、毛壳菌属(Chaetomium)和周刺座霉属(Volutella),绿腐植株根际土中的优势微生物为节杆菌属(Arthrobacter)和短梗蠕孢属(Trichocladium)。黄腐植株根内优势微生物为被孢霉属(Mortierella)和木霉属(Trichoderma),绿腐植株根内优势微生物为假单胞菌属(Pseudomonas)、土赤壳菌属(Ilyonectria)和小不整球壳属(Plectosphaerella)。[结论]三七根腐病的发生与植株根际及根内微生物多样性和群落结构密切相关。[Objective]To study the microbial community structure in the root and rhizosphere soil of healthy and root rot infected plants of Panax notoginseng,and explore the correlation between microbial diversity with root rot of P.notoginseng.[Method]The microbial community composition and diversity of the roots and rhizosphere soil of healthy and root rot plants in Wenshan,Yunnan Province,which had been continuously cultivated for 3 years were analyzed by using Illumina high-throughput sequencing system.[Result]The richness of microbial communities in healthy plants roots and rhizosphere soil was significantly different from that in root rot plants roots and rhizosphere soil.The dominant microorganisms in the rhizosphere soil of yellow rot plants were Arthrobacter,Chaetomium and Volutella,and that of the rhizosphere soil of green rot plant were Arthrobacter and Trichocladium.The dominant microorganisms in roots of yellow rot plants were Mortierella and Trichoderma,while the dominant microorganisms in roots of green rot plants were Pseudomonas,Ilyoectria and Plectosphaerella.[Conclusion]The occurrence of root rot in Panax notoginseng is closely related to the diversity and community structure of microorganisms in the rhizosphere and roots of plants.

关 键 词：三七 根腐病 根际微生物 高通量测序 群落结构 微生物多样性 

分 类 号：X172[环境科学与工程—环境科学]