Complement activation targeted inhibitor C2-FH ameliorates acetaminophen-induced liver injury in mice  

作　　者：Chun-Mei Li Tian Sun Mou-Jie Yang Zhi Yang Qing Li Jia-Lin Shi Chong Zhang Jun-Fei Jin 

机构地区：[1]Guangxi Key Laboratory of Molecular Medicine in Liver Injury and Repair,the Affiliated Hospital of Guilin Medical University,Guilin 541001,Guangxi Zhuang Autonomous Region,China [2]Guangxi Health Commission Key Laboratory of Basic Research in Sphingolipid Metabolism Related Diseases,the Affiliated Hospital of Guilin Medical University,Guilin 541001,Guangxi Zhuang Autonomous Region,China [3]China-United States Lipids in Health and Disease Research Center,Guilin Medical University,Guilin 541001,Guangxi Zhuang Autonomous Region,China [4]Laboratory of Hepatobiliary and Pancreatic Surgery,the Affiliated Hospital of Guilin Medical University,Guilin 541001,Guangxi Zhuang Autonomous Region,China

出　　处：《World Journal of Hepatology》2024年第10期1188-1198,共11页世界肝病学杂志（英文）

基　　金：Supported by Natural Science Foundation of Guangxi,No.2020GXNSFDA238006;Special Fund of the Central Government Guiding Local Scientific and Technological Development by Guangxi Science and Technology Department,No.GuikeZY21195024;Research Enhancement Project for Junior Faculty in Higher Education Institutes of Guangxi,No.2018KY0419.

摘　　要：BACKGROUND Complement activation is recognized as an important factor in the progression of liver damage caused by acetaminophen(APAP).However,the role of the complement inhibitor C2-FH in APAP-induced liver injury remains unclear.AIM To explore C2-FH in protecting against APAP-induced liver injury by inhibiting complement activation.METHODS A model of APAP-induced liver injury was used to study the protective effect of C2-FH on liver injury.C2-FH was administered through intraperitoneal injection 30 minutes after APAP treatment.We detected the effects of C2-FH on liver function,inflammatory response and complement activation.Additionally,RNA-sequencing(RNA-Seq)analysis was conducted to understand the mechanism through which C2-FH provides protection against APAP-induced liver injury.RESULTS C2-FH inhibited the increase in serum alanine aminotransferase activity,aspartate aminotransferase activity and lactate dehydrogenase,and reduced liver tissue necrosis caused by APAP.Moreover,it attenuated the inflammatory response and inhibited complement activation in APAP-induced liver injury.RNA-Seq analysis provided additional explanations for the protective role of C2-FH against APAP-induced liver injury.CONCLUSION C2-FH attenuates APAP-induced liver injury by inhibiting complement activation.

关 键 词：C2-FH COMPLEMENT Complement activation Acetaminophen-induced liver injury Inflammation 

分 类 号：R575[医药卫生—消化系统]