桃胶多糖对便秘模型小鼠的改善作用研究  

Improving effect of peach gum polysaccharide on constipation model mice

作　　者：高帆 苏洁[1] 周衡朴 李进 应国伟 陈素红[2] 吕圭源[1] GAO Fan;SU Jie;ZHOU Hengpu;LI Jin;YING Guowei;CHEN Suhong;LYU Guiyuan(School of Pharmaceutical Sciences,Zhejiang Chinese Medical University,Hangzhou 310053,China;College of Pharmaceutical Science,Zhejiang University of Technology,Hangzhou 310014,China)

机构地区：[1]浙江中医药大学药学院,浙江杭州310053 [2]浙江工业大学药学院,浙江杭州310014

出　　处：《食品与发酵工业》2024年第19期40-48,共9页Food and Fermentation Industries

基　　金：浙江省重点研发计划项目(2020C04020)。

摘　　要：该文探讨桃胶多糖(peach gum polysaccharide,PGP)缓解便秘的作用及机制。采用盐酸洛派丁胺(10 mg/kg BW)连续灌胃14 d建立便秘模型小鼠,模型成功后给予桃胶多糖(100、300 mg/kg)连续3周。给药期间测定小鼠6小时内粪便粒数、粪便含水率、首次黑便时间、肠道推进率、胃排空率和行为学指标;ELISA法测定血清中胃泌素(gastrin,GAS)、胃动素(motilin,MTL)和5-羟色胺(5-hydroxytryptamine,5-HT)含量;阿利新蓝-过碘酸-雪夫染色(Alixin blue periodic acid Schiff staining,AB-PAS)染色法观察结肠组织中杯状细胞数目;H&E染色法观察结肠组织形态;免疫组化法观察小鼠结肠组织中5-羟色胺受体3(5-hydroxytryptamine receptor 3,5-HT3R)表达;免疫荧光法观察小鼠结肠组织中闭合蛋白(occludin)和紧密连接蛋白-1(claudin-1)表达;Western blot法测定结肠组织中Occludin、5-HT3R和色氨酸羟化酶1(tryptophan hydroxylase 1,TPH-1)相对表达量。桃胶可以显著增加便秘模型小鼠的抓力、自主活动次数、开放臂进入次数和开放臂停留时间;增加粪便粒数粪便含水率和胃排空率,缩短首次黑便时间;升高血清中GAS和MTL水平;增加结肠组织中杯状细胞数量,改善肠粘膜损伤,并上调Occludin、Claudin-1蛋白表达;增加血清中5-HT水平及结肠组织中5-HT3R和TPH-1蛋白表达。桃胶多糖具有改善便秘的作用,其机制可能与促进胃肠激素分泌和激动5-HT信号通路有关。To investigate the effect and mechanism of Peach gum polysaccharide(PGP)in relieving constipation.Constipation model mice were established by continuous intragastric administration of loperamide hydrochloride(10 mg/kg BW)for 14 days,and peach gum polysaccharides(100 and 300 mg/kg)were given for 3 weeks after the model was successful.The number of fecal grains,fecal water content,first black stool time,intestinal propulsion rate,gastric emptying rate and behavioral indexes were measured within 6 hours during administration.The contents of Gastrin(GAS),Motilin(MTL)and 5-hydroxytryptamine(5-HT)in serum were determined by ELISA.The number of goblet cells in colon tissues was observed by Alcian blue-periodate Scheff(AB-PAS)staining.The colonic morphology was observed by H&E staining.The expression of 5-HT3R(5-HT3R)in colon tissue of mice was observed by immunohistochemistry.The expression of tight junction proteins Occludin and Claudin-1 in colon tissue of mice was observed by immunofluorescence method.The relative expression of Occludin,5-HT3R and tryptophan hydroxylase 1(TPH-1)in colon tissue was determined by Western blot.Peach gum can significantly increase the grasping power,the number of autonomous activities,the number of open arm entry and the stay time of open arm in mice with constipation model.Increase fecal grain count,fecal moisture content and gastric emptying rate,shorten the time of first black stool;Increase the levels of GAS and MTL in serum;Increase the number of goblet cells in colon tissue,improve intestinal mucosal injury,and up-regulate the expression of Occludin and Claudin-1 proteins;The level of 5-HT in serum and the expression of 5-HT3R and TPH-1 protein in colon tissue were increased.Peach gum polysaccharide can ameliorate constipation,and its mechanism may be related to promoting gastrointestinal hormone secretion and activating 5-HT signaling pathway.

关 键 词：桃胶多糖 便秘 5-HT 胃肠激素 OCCLUDIN 

分 类 号：TS201.2[轻工技术与工程—食品科学] TS201.4[轻工技术与工程—食品科学与工程] R574.62[医药卫生—消化系统]