基于靶向代谢组学分析不同花色大红花花青素的差异  

Analysis on Differences in Anthocyanins of Hibiscus rosa-sinensi with Different Flower Colors Based on Targeted Metabolomics

作　　者：桑贤东 杨晓慧[1] 徐斌[1] 余少雄 张卫华[1] 廖焕琴 杨会肖[1] SANG Xiandong;YANG Xiaohui;XU Bin;YU Shaoxiong;ZHANG Weihua;LIAO Huanqin;YANG Huixiao(Guangdong Academy of Forestry/Guangdong Key Laboratory of Silviculture,Protection and Utilization,Guangzhou 510520,China;Shanwei Public Utility Affairs Center,Shanwei 516600,China)

机构地区：[1]广东省林业科学研究院/广东省森林培育与保护利用重点实验室,广东广州510520 [2]汕尾市公用事业事务中心,广东汕尾516600

出　　处：《广东农业科学》2024年第8期61-70,共10页Guangdong Agricultural Sciences

基　　金：汕尾市省科技专项(220130105850566);广东省自然科学基金(2214050006239)。

摘　　要：【目的】大红花(Hibiscus rosa-sinensis L.)因花色丰富、观赏价值较高,被广泛应用于华南地区的城市绿化与园林造景中。研究大红花不同花色中花青素种类及含量的特征差异,分析大红花不同品种间代谢物特异性及共同性特征,为深入挖掘品种特性及花色定向育种提供理论和技术支撑。【方法】利用靶向代谢组学技术对白色(W)、红色(R)、黄色(Y)3种花色大红花花瓣组织中的花青素苷组成和含量进行测定,利用通路富集分析及多元统计学方法对代谢物进行功能注释、鉴定与分析。【结果】大红花W、R与Y花色中共检测出90种代谢物,其中差异代谢物共有78种,主要为矢车菊素(41.11%)、飞燕草素(20.00%)、芍药花素(13.33%)等。不同花色大红花间差异代谢物分别为62个(YvsR)、50个(YvsW)和65个(RvsW),其中共有差异代谢物22个。综合考虑各个比较组合差异显著性和绝对含量,发现矢车菊素-3-O-桑布双糖苷、天竺葵素-3-O-槐糖苷、矢车菊素-3-O-龙胆二糖苷和芍药花素-3-O-葡萄糖苷含量在红色花中较高;飞燕草素-3-O-(6''-O-酒石酰)葡萄糖苷、原花青素B4和原花青素B3含量在黄色花中较高。KEGG代谢通路分析发现差异代谢物在不同花色的组织中显著富集。【结论】矢车菊素-3-O-桑布双糖苷、天竺葵素-3-O-槐糖苷、矢车菊素-3-O-龙胆二糖苷和芍药花素-3-O-葡萄糖苷是大红花呈红色的重要代谢物,原花青素B3是花色呈黄色的重要代谢物。该结果初步揭示大红花不同花色的代谢物差异,可为大红花花色形成机制和花色改良提供理论参考。【Objective】Hibiscus rosa-sinensis L.has high ornamental value due to rich flower colors and is widely used in urban greening and landscape architecture in South China.In order to provide theoretical and technical support for in-depth excavation of variety characteristics and flower color directional breeding technology,the characteristic differences of types and contents of anthocyanin in different flower colors were detected,and the specificity and common characteristics of different varieties of H.rosa-sinensis L.were analyzed.【Method】The anthocyanin composition and content of different petal tissues of white(W),red(R)and yellow(Y)flowers were determined by targeted metabolomics.Functional annotation,identification and analysis of metabolites were performed by using pathway enrichment analysis and multivariate statistical methods.【Result】The results showed that a total of 90 metabolites were detected in W,R and Y colors of H.rosa-sinensis L.,among which 78 were differential metabolites,including cyanidin(41.11%),delphinidin(20.00%),peonidin(13.33%),and etc.There were 62(Y vs R),50(Y vs W)and 65(R vs W)differential metabolites among different flower colors,with a total of 22 common differential metabolites.Comprehensively considering the significance and absolute contents of differences in different comparative combinations,it was found thta the cyanidin-3-O-sambubioside-glucoside,pelargonidin-3-O-sophoroside,cyanidin-3-O-gentiobioside and peonidin-3-O-glucoside were relatively high in red flower;the delphinidin-3-O-(6''-Otartaryl)glucoside,procyanidin B4 and procyanidin B3 were relatively high in yellow flower.KEGG metabolic pathways analysis revealed that differential metabolites were significantly enriched in different flower color tissues.【Conclusion】The cyanidin-3-O-sambubioside-glucoside,pelargonidin-3-O-sophoroside,cyanidin-3-O-gentiobioside and peonidin-3-O-glucoside were important metabolites determining the flower red color,while procyanidin B3 was an important metabolite determining th

关 键 词：大红花 花色 代谢组学 花青素 合成通路 超高效液相色谱-串联质谱 

分 类 号：S793.9[农业科学—林木遗传育种]