低温等离子体诱导红小豆萌发富集γ-氨基丁酸的工艺优化及相关酶活性变化  

Process Optimization of Low-temperature Plasma-Induced Germination Enrichment ofγ-Aminobutyric Acid in Adzuki Beans and Changes in Related Enzyme Activities

作　　者：许庆鹏 姜秀杰[1] 张家瑜 周航庆 张东杰[1] Xu Qingpeng;Jiang Xiujie;Zhang Jiayu;Zhou Hangqing;Zhang Dongjie(College of Food Science,Heilongjiang Bayi Agricultural University,Daqing163319)

机构地区：[1]黑龙江八一农垦大学食品学院,大庆163319

出　　处：《黑龙江八一农垦大学学报》2024年第5期77-86,共10页journal of heilongjiang bayi agricultural university

基　　金：黑龙江省“百千万”工程科技重大专项(2021ZX12B06);大庆市指导性科技计划项目(zd-2021-73);黑龙江省自然科学基金研究团队项目(TD2020C003);黑龙江八一农垦大学“三纵”基础培育项目(ZRCPY202122)。

摘　　要：为研究等离子体处理红小豆种子对其萌发富集GABA的作用,以红小豆为原料,选择大气压低温等离子处理及恒温恒湿条件下培养红小豆萌发。在单因素的基础上以等离子体时间、电压、频率和发芽时间为响应因子,以GABA含量为响应值,采用Design-Expert 13软件对红小豆萌发富集GABA工艺进行优化,并对萌发阶段GABA合成关键限速酶活性进行研究。结果显示,等离子体胁迫红小豆萌发富集GABA最佳工艺为等离子体时间30 min、等离子体电压90 kV、等离子体频率130 Hz、发芽时间60 h,此条件下GABA含量最高为114.45±1.08 mg·100 g^(-1),是未发芽的4.53倍。此外,发现在萌发阶段等离子体处理的红小豆GAD酶活显著高于原料红小豆,表明低温等离子体胁迫红小豆萌发富集GABA是通过增加GABA支路的GAD酶活实现,而AMADH酶活显著低于原料红小豆,说明多胺降解途径对GABA积累作用较小。In order to study the effect of plasma treatment on the germination and enrichment of adzuki bean seeds,adzuki beans were used as raw materials,atmospheric pressure cold plasma treatment and cultivation of adzuki beans under constant temperature and humidity conditions.On the basis of single factors,the GABA process of adzuki bean germination and enrichment was optimized by Design-Expert 13 software,and the activity of GABA synthesis in the germination stage was studied,using plasma time,plasma voltage,plasma frequency and germination time as response factors,and GABA content as the response value.The results showed that the optimal process of plasma stress adzuki bean germination and enrichment process was as follows:plasma time 30 min,plasma voltage 90 kV,plasma frequency 130 Hz,germination time 60 h,and the highest GABA content under this condition was 114.45±1.08 mg·100 g^(-1).In addition,it was found that the GAD enzyme activity of plasma-treated adzuki beans at the germination stage was higher than that of normal adzuki beans,while the overall AMADH enzyme activity was lower than that of normal adzuki beans.

关 键 词：大气压低温等离子 红小豆 Γ-氨基丁酸 酶活 

分 类 号：TS201.2[轻工技术与工程—食品科学]