草原苏尼特羊脑中磷脂型DHA萃取及其结构特性研究  

Extraction and structural characteristics of phospholipid DHA from grassland Sunite sheep brain

作　　者：包音都古荣·金花[1,2,3] 和硕特麦丽斯 呼格吉勒图 侯荣伦 于波 周欢敏 BAOYINDUCURONG·Jinhuat;HESHUOTE Mailisi;HUGEJILETU;HOU Ronglun;YU Bo;ZHOU Huanmi(College of Food Science and Engincering,Inner Mongolia Agricultural University,Hohhot 010010,China;Inner Mongolia Key Laboratory of Biofabrication,Hohbot 010010,China;Inner Mongolia Hesigr Agriculture and Animal Husbandry Technology Co.,Ltd.,Xilingol League 026321,Inner Mongolia,China;Crystal Pharmatech,Inc.,Cranbury,NJ 08512,USA)

机构地区：[1]内蒙古农业大学食品科学与工程学院,呼和浩特010010 [2]内蒙古生物制造重点实验室,呼和浩特010010 [3]内蒙古贺斯格农牧业科技有限公司,内蒙古锡林郭勒盟026321 [4]晶云药物科技股份有限公司,美国新泽西08512

出　　处：《中国油脂》2024年第10期34-39,61,共7页China Oils and Fats

基　　金：内蒙古自治区自然科学基金项目(2023MS03034);内蒙古自治区科技计划项目(2023YFDZ0050);内蒙古自治区科技计划项目(2021GG0348);草原英才项目(内组通字〔2021〕333号,内人才发〔2023〕3号)。

摘　　要：旨在为绿色天然草原羊脑中磷脂型二十二碳六烯酸(DHA-PL)的开发利用提供科学数据,以苏尼特冻干羊脑为原料,采用溶剂萃取其中的粗脂肪,用冰丙酮沉淀得到磷脂,再采用硅胶柱层析法分离其中的磷脂酰胆碱(PC)和磷脂酰丝氨酸(PS)。测定了粗脂肪中胆固醇含量,以及磷脂中PC和PS的含量,分析了粗脂肪、磷脂、PC和PS中DHA含量,以及PC和PS中sn-2位的DHA含量。结果表明:采用体积比2∶1乙醇-正己烷萃取冻干苏尼特羊脑中粗脂肪,粗脂肪得率较高,为45%;羊脑粗脂肪中胆固醇含量为29.42mg/g,粗脂肪的脂肪酸组成中DHA含量为8.89%;磷脂的脂肪酸组成中DHA含量为11.41%;磷脂中PC和PS含量分别为27.67μg/g和10.69μg/g,PC和PS的sn-2位脂肪酸组成中DHA含量分别为8.06%和2.59%。综上,可以从苏尼特羊脑中制备易于吸收的DHA-PL。To provide scientific data for the development and utilization of phospholipid docosahexaenoic acid(DHA-PL)in green natural grassland sheep brain,the crude fat was extracted with solvent from the freeze-dried Sunite sheep brain and precipitated with ice acetone to obtain phospholipid,and then the phosphatidylcholine(PC)and phosphatidylserine(PS)were separated by silica gel column chromatography from phospholipid.The total cholesterol content in the crude fat was determined,as well as the PC and PS contents in the phospholipid.The contents of DHA in crude fat,phospholipid,PC and PS,and the contents of DHA at sn-2 position in PC and PS were analyzed.The results showed that the higher crude fat yield of 45%was obtained by extraction with ethanol-n-hexane(volume ratio of 2∶1).The cholesterol content in the crude fat was 29.42 mg/g,and the DHA content in the crude fat was 8.89%.The DHA content in the phospholipid was 11.41%.The contents of PC and PS in phospholipid were 27.67μg/g and 10.69μg/g,respectively.The DHA content in the sn-2 position of PC and PS was 8.06%and 2.59%,respectively.In summary,DHA-PL that is easily absorbed can be prepared from the Sunite sheep brain.

关 键 词：草原苏尼特羊脑 磷脂型DHA sn-2位脂肪酸 脂肪酸组成 DHA含量 

分 类 号：TS225.6[轻工技术与工程—粮食、油脂及植物蛋白工程] TQ646[轻工技术与工程—食品科学与工程]