氧化κ-卡拉胶/聚丙稀酰胺水凝胶的制备与表征  

Preparation and haracterization of oxidizedκ-carrageenan/polyacrylamide hydrogel

作　　者：李招玉 李向平 杨少玲[2] 潘创 李春生[2] 胡晓[2] 赵永强[2] 邓建朝[2] 戚勃[2] LI Zhaoyu;LI Xiangping;YANG Shaoling;PAN Chuang;LI Chunsheng;HU Xiao;ZHAO Yongqiang;DENG Jianchao;QI Bo(College of Food Science and Technology,Shanghai Ocean University,Shanghai 201306,China;South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Key Laboratory of Aquatic Product Processing,Guangzhou 510300,China;Affiliated TCM Hospital of Guangzhou Medical University,Guangzhou 510130,China)

机构地区：[1]上海海洋大学食品学院,上海201306 [2]中国水产科学研究院南海水产研究所,农业农村部水产品加工重点实验室,广东广州510300 [3]广州医科大学附属中医医院,广东广州510130

出　　处：《食品与发酵工业》2024年第20期153-160,共8页Food and Fermentation Industries

基　　金：国家现代农业产业技术体系(CARS-50);中国水产科学研究院中央级公益性科研院所基本科研业务费专项资金(2023TD74);中国水产科学研究院南海水产研究所中央级公益性科研院所基本科研业务费(2021SD06)。

摘　　要：为提高κ-卡拉胶水凝胶的应用性能,该文以κ-卡拉胶(κ-carrageenan, KC)为原料,通过TEMPO/NaClO/NaBr(TEMPO:2,2,6,6-四甲基哌啶-1-氧基自由基,2,2,6,6-tetramethylpiperidoxyl)反应体系制备氧化κ-卡拉胶(oxidizedκ-carrageenan, OKC),再用OKC与聚丙烯酰胺(polyacrylamide, PAM)以不同质量比进行复配,采用化学交联法制备了氧化κ-卡拉胶/聚丙烯酰胺水凝胶,通过测定水凝胶的含水率、水溶性、溶胀率、机械性能、红外光谱和微观结构,探究了氧化κ-卡拉胶对水凝胶应用性能的影响。结果表明,OKC可显著提高复合水凝胶的溶胀率、拉伸强度和断裂伸长率、降低水溶性(P<0.05),且当OKC与AM质量比为3∶4时,复合水凝胶的性能指标达最优值,即OKC3/PAM4含水率为82.7%,水溶性为12.2%,溶胀率为887%,拉伸强度达0.223 MPa,断裂伸长率达448%;红外光谱分析显示,κ-卡拉胶中的C-6醇羟基成功氧化为C-6羧基,氧化κ-卡拉胶/聚丙烯酰胺水凝胶各组分之间存在氢键;扫描电镜显示,OKC/PAM水凝胶具有致密多孔的三维网状结构,孔径均匀、规整。因此,氧化κ-卡拉胶与聚丙烯酰胺复配可制备成性能良好的水凝胶,将为开发卡拉胶水凝胶医用敷料提供理论基础。κ-Carrageenan is a macromolecular polysaccharide substance with galactose and dehydrated galactose as the main chain.It is also one of the most important edible gums.It can be used to prepare composite hydrogel with other hydrophilic polymers and has important application potential in the field of medical materials.To improve the application performance of κ-carrageenan hydrogel,in this paper,oxidized κ-carrageenan(OKC)was prepared by TEMPO/NaClO/NaBr reaction system using κ-carrageenan(KC)as the material,and then the oxidized κ-carrageenan/polyacrylamide hydrogel was prepared by chemical crosslinking method using OKC and polyacrylamide(PAM)in different mass ratio.The effect of oxidized κ-carrageenan on the application properties of hydrogel was investigated by measuring moisture content,water solubility,swelling rate,mechanical properties,infrared spectroscopy,and microstructure of hydrogel.Results showed that OKC could significantly increase the swelling rate,tensile strength,and elongation at break,and decrease the water solubility of the composite hydrogel(P<0.05),and when the ratio of OKC/PAM was 3∶4,the performance index of the composite hydrogel reached the optimal value,the moisture content of OKC_(3)/PAM_(4) was 82.7%,the water solubility was 12.2%,the swelling rate was 887%,the tensile strength reached 0.223 MPa,and the elongation at break reached 448%.Infrared spectroscopy analysis showed that the C-6 alcohol hydroxyl group in κ-carrageenan was oxidized to the C-6 carboxyl group,and hydrogen bonding existed between the components of the oxidized κ-carrageenan/polyacrylamide hydrogel.Scanning electron microscope showed that the OKC/PAM hydrogel had a dense porous three-dimensional network structure with uniform and regular pore size.Therefore,OKC and PAM can be compounded to prepare hydrogel with good performance,which will provide a theoretical basis for the development of carrageenan hydrogel medical dressing.

关 键 词：Κ-卡拉胶 氧化κ-卡拉胶 聚丙烯酰胺 水凝胶 

分 类 号：TS2[轻工技术与工程—食品科学与工程]