Abalone shell-derived Mg-doped mesoporous hydroxyapatite microsphere drug delivery system loaded with icariin for inducing apoptosis of osteosarcoma cells  

作　　者：Kaihua Liu Meiqi Cheng Hao Huang Hui Yu Shiyao Zhao Jinnuo Zhou Dan Tie Jianhua Wang Panpan Pan Jingdi Chen 

机构地区：[1]Marine College,Shandong University,Weihai,Shandong Province,China [2]State Key Laboratory of Mineral Processing,Beijing,China [3]College of Biological Science and Technology,Fuzhou University,Fuzhou,Fujian Province,China

出　　处：《Biomaterials Translational》2024年第2期185-196,共12页生物材料转化电子杂志（英文）

基　　金：supported by Open Foundation of State Key Laboratory of Mineral Processing(No.BGRIMM-KJSKL-2023-23);Shandong Laboratory of Advanced Materials and Green Manufacturing(No.AMGM2021F02);Natural Science Foundation of Shandong Province(Nos.ZR2022QD057,ZR2023MC125);Open Project Fund for Hubei Key Laboratory of Oral and Maxillofacial Development and Regeneration(No.2021kqhm003);China Postdoctoral Science Foundation(Nos.2022M722434,2023T160492).

摘　　要：Hydroxyapatite(HAP)porous microspheres with very high specific surface area and drug loading capacity,as well as excellent biocompatibility,have been widely used in tumour therapy.Mg^(2+)is considered to be a key factor in bone regeneration,acting as an active agent to stimulate bone and cartilage formation,and is effective in accelerating cell migration and promoting angiogenesis,which is essential for bone tissue repair,anti-cancer,and anti-infection.In this study,abalone shells from a variety of sources were used as raw materials,and Mg^(2+)-doped abalone shell-derived mesoporous HAP microspheres(Mg-HAP)were prepared by hydrothermal synthesis as Mg^(2+)/icariin smart dual delivery system(ICA-Mg-HAP,IMHA).With increasing of Mg^(2+)doping,the surface morphology of HAP microspheres varied from collapsed macroporous to mesoporous to smooth and non-porous,which may be due to Mg^(2+)substitution or coordination in the HAP lattice.At 30%Mg^(2+)doping,the Mg-HAP microspheres showed a more homogeneous mesoporous morphology with a high specific surface area(186.06 m^(2)/g).The IMHA microspheres showed high drug loading(7.69%)and encapsulation rate(83.29%),sustained Mg^(2+)release for more than 27 days,sustained and stable release of icariin for 60 hours,and good responsiveness to pH(pH 6.4>pH 5.6).In addition,the IMHA delivery system stimulated the rapid proliferation of bone marrow mesenchymal stem cells and induced apoptosis in MG63 cells by blocking the G2 phase cycle of osteosarcoma cells and stimulating the high expression of apoptotic genes(Bcl-2,caspase-3,-8,-9).This suggests that the abalone shell-based IMHA may have potential applications in drug delivery and tumour therapy.

关 键 词：abalone shell drug delivery hydroxyapatite microspheres magnesium doping OSTEOSARCOMA 

分 类 号：R738.1[医药卫生—肿瘤]