辣木核桃乳的研制及其对小鼠睡眠改善作用  

Development of Moringa oleifera Walnut Milk and Its Effect on Sleep Improvement in Mice

作　　者：吴宽 牟泓羽 杨瑜芳 余智瑾 盛军[1,3] 赵存朝 WU Kuan;MOU Hongyu;YANG Yufang;YU Zhijin;SHENG Jun;ZHAO Cunchao(School of Food Science and Technology,Yunnan Agricultural University,Kunming 650201,Yunnan,China;Kunming Institute of Biological Manufacturing Co.,Ltd.,Kunming 650201,Yunnan,China;Yunnan Plateau Characteristic Agricultural Industry Research Institute,Kunming 650201,Yunnan,China)

机构地区：[1]云南农业大学食品科学技术学院,云南昆明650201 [2]昆明生物制造研究院有限公司,云南昆明650201 [3]云南省高原特色农业产业研究院,云南昆明650201

出　　处：《食品研究与开发》2024年第20期93-99,共7页Food Research and Development

基　　金：云南省科技厅科技计划项目(202102AE090027)。

摘　　要：该文以去皮核桃仁和辣木叶发酵提取物为主要原料,通过感官评价和响应面工艺优化,研制一款风味辣木核桃乳,并通过对小鼠直接睡眠实验、延长戊巴比妥钠诱导的小鼠睡眠实验、戊巴比妥钠阈下剂量睡眠实验和巴比妥钠睡眠潜伏期实验,对其睡眠改善作用进行探究。结果表明:辣木核桃乳最佳工艺配方为辣木叶发酵提取物添加量16 g/kg、核桃仁添加量80 g/kg、白砂糖添加量50 g/kg,该配方下得到的辣木核桃乳口感最好,感官评分达93.2;辣木核桃乳对小鼠无直接睡眠作用;与空白对照组相比,辣木核桃乳低、中、高剂量组均对戊巴比妥钠催眠诱导的小鼠睡眠时长有积极影响,分别延长10.28%、33.04%、61.11%;辣木核桃乳中剂量组显著提高小鼠的睡眠发生率(p<0.05),辣木核桃乳高剂量组极显著提高小鼠的睡眠发生率(p<0.01);辣木核桃乳中剂量组显著缩短巴比妥钠诱导的小鼠睡眠潜伏期(p<0.05),辣木核桃乳高剂量组极显著缩短小鼠的睡眠潜伏期(p<0.01)。综上,该辣木核桃乳口感细腻、风味独特,对小鼠的睡眠具有明显改善作用。By means of sensory evaluation and process optimization of response surface,this study focused on the development of a flavored Moringa oleifera walnut milk beverage using peeled walnut kernels and fer⁃mented M.oleifera leaves extracts as primary ingredients The beverage's efficacy in improving sleep was inves⁃tigated using direct sleep tests in mice,extended pentobarbital sodium⁃induced sleep tests,sub⁃threshold doses of pentobarbital sodium for sleep induction,and barbiturate sodium sleep latency tests.The results indicated that the optimal formulation of the beverage consisted of 16 g/kg of M.oleifera leaves fermented extract,80 g/kg of walnut kernel,and 50 g/kg of white granulated sugar.This composition provided the best taste,achieving a sensory score of 93.2.The beverage did not induce sleep directly in mice;however,compared to the control group,low,medium,and high doses of the beverage significantly increased the duration of sleep induced by pentobarbital sodium by 10.28%,33.04%,and 61.11%,respectively.Additionally,the medium dose signifi⁃cantly enhanced the occurrence rate of sleep(p<0.05),and the high dose significantly increased this rate fur⁃ther(p<0.01).The medium dose significantly reduced the latency period of sleep induced by barbiturate so⁃dium(p<0.05),while the high dose had a highly significant effect in reducing the sleep latency period(p<0.01).In conclusion,the beverage,characterized by its delicate mouthfeel and unique flavor,could signifi⁃cantly improve sleep in mice.

关 键 词：核桃 辣木 发酵 改善睡眠 小鼠 

分 类 号：TS275.4[轻工技术与工程—农产品加工及贮藏工程] TS201.4[轻工技术与工程—食品科学与工程]