Sandpaper-templated Stretchable Immunosensing Electrodes for Sub-picomolar Progesterone Detection  

作　　者：LI Zhaoxian MENG Xingyu FANG Chuyao YI Zhenkai WU Yaoyao LIU Xuanxuan ZHONG Wei ZHANG Limei XIE Zhuang 

机构地区：[1]School of Materials Science and Engineering,Key Laboratory for Polymeric Composite and Functional Materials of Ministry of Education,Sun Yat-sen University,Guangzhou,510275,P.R.China [2]Reproductive Medicine Center,Department of Obstetrics and Gynecology,the First Affiliated Hospital of Sun Yat-sen University,Guangzhou,510080,P.R.China

出　　处：《Chemical Research in Chinese Universities》2024年第5期874-880,共7页高等学校化学研究（英文版）

基　　金：supported by the National Natural Science Foundation of China(No.22075325);the Fundamental Research Funds for the Central Universities,Sun Yat-sen University,China(No.23ptpy51).

摘　　要：Female hormone detection,particularly non-invasive monitoring progesterone(P4)levels in body fluids,plays a critical role in female health management and disease diagnosis.However,the challenge still exists because of the ultralow abundance of P4(<100 pmol/L)in sweat and saliva,necessitating highly sensitive methods for wearable detection.Herein,we present a simple sandpaper-templated stretchable immunosensing electrode designed for ultra-sensitive detection of P4 at sub-picomolar level.A molding technique is employed to replicate the sandpaper textures to provide a microstructured elastomeric substrate for electrode preparation.Such microstructured surface coated with poly(3,4-ethylenedioxythiophene):poly(4-styrenesulfonate)(PEDOT:PSS)/LiTFSI[LiTFSI=lithium bis(trifluoromethanesulfonyl)imide]provided a stretchable polymer electrode with high conductivity,and further decoration with gold nanoparticles(AuNPs)enabled the immunosensing for P4 by electrochemical impedance spectroscopy(EIS)measurements.Through adjusting the AuNPs deposition conditions,ultrasensitive detection of P4 is realized with a low limit of detection(LOD)of ca.10 fmol/L and a tunable dynamic range up toµmol/L.Notably,the stretchable electrode exhibits stable electrochemical performance,enabling the detection of P4 at sub-picomolar levels even under mechanical strain of 30%.This innovative electrochemical sensor holds significant promise for non-invasive,on-site monitoring of P4 levels in healthcare,as well as hormone detection in food safety and environment surveillance.

关 键 词：PROGESTERONE IMMUNOSENSOR Microstructured electrode Stretchable biosensor Electrochemical sensing 

分 类 号：R318[医药卫生—生物医学工程]