Enhancement of polymyxin B1 production by an artificial microbial consortium of Paenibacillus polymyxa and recombinant Corynebacterium glutamicum producing precursor amino acids  

作　　者：Hui-Zhong Sun Si-Yu Wei Qiu-Man Xu Wei Shang Qing Li Jing-Sheng Cheng Ying-Jin Yuan 

机构地区：[1]Frontiers Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering(Ministry of Education),School of Chemical Engineering and Technology,Tianjin University,Yaguan Road 135,Jinnan District,Tianjin,300350,PR China [2]Tianjin Key Laboratory of Animal and Plant Resistance,College of Life Science,Tianjin Normal University,Binshuixi Road 393,Xiqing District,Tianjin,300387,PR China [3]Department of Pharmaceutical,School of Chemical Engineering and Technology,Tianjin University,Yaguan Road 135,Jinnan District,Tianjin,300350,PR China

出　　处：《Synthetic and Systems Biotechnology》2024年第1期176-185,共10页合成和系统生物技术（英文）

基　　金：grateful for the financial supports from the National Key R&D Program of China(2018YFA0902200);the National Natural Science Foundation of China(Program:21878224).

摘　　要：Polymyxin B,produced by Paenibacillus polymyxa,is used as the last line of defense clinically.In this study,exogenous mixture of precursor amino acids increased the level and proportion of polymyxin B1 in the total of polymyxin B analogs of P.polymyxa CJX518-AC(PPAC)from 0.15 g/L and 61.8%to 0.33 g/L and 79.9%,respectively.The co-culture of strain PPAC and recombinant Corynebacterium glutamicum-leu01,which produces high levels of threonine,leucine,and isoleucine,increased polymyxin B1 production to 0.64 g/L.When strains PPAC and C.glu-leu01 simultaneously inoculated into an optimized medium with 20 g/L peptone,polymyxin B1 production was increased to 0.97 g/L.Furthermore,the polymyxin B1 production in the co-culture of strains PPAC and C.glu-leu01 increased to 2.21 g/L after optimized inoculation ratios and fermentation medium with 60 g/L peptone.This study provides a new strategy to improve polymyxin B1 production.

关 键 词：POLYMYXIN CO-CULTURE Paenibacillus polymyxa Corynebacterium glutamicum Medium optimization Metabolic precursors 

分 类 号：Q93[生物学—微生物学]