气相色谱法检测橄榄油中反式脂肪酸含量的方法学研究  

Methodological Study on the Detection of Trans Fatty Acid Content in Olive Oil by Gas Chromatography

作　　者：彭万才 唐顺之[1] 李遥 魏劭恒 李佳俐 许文东[1] PENG Wancai;TANG Shunzhi;LI Yao;WEI Shaoheng;LI Jiali;XU Wendong(National Engineering Research Center of Pharmaceutical Processing Technology of Traditional Chinese Medicine and Drug Innovation,Guangzhou Hanfang Pharmaceutical Co.,Ltd.,Guangdong Provincial Key Laboratory of Medicinal Lipid,Guangzhou 510240,China)

机构地区：[1]中药制药过程技术与新药创制国家工程研究中心,广州白云山汉方现代药业有限公司,广东省药用脂质重点实验室,广东广州510240

出　　处：《食品安全导刊》2024年第29期79-83,89,共6页China Food Safety Magazine

基　　金：广东省药用脂质重点实验室(2023B1212070030)。

摘　　要：目的:建立气相色谱法检测橄榄油中10种反式脂肪酸的方法并进行方法学验证。方法:样品经甲酯化后,以Supelco SP-2560毛细管柱为分析柱,经程序升温和氢火焰离子化检测器进行检测。结果:橄榄油中10种反式脂肪酸能实现良好分离,未见其他成分干扰,方法的检测限和定量限、精密度和回收率、耐用性、专属性均良好。结论:该方法准确可靠、简单易行,可用于橄榄油中反式脂肪酸含量检测。Objective:To establish a gas chromatography method for the determination of 10 trans-fatty acids in olive oil and to verify the method.Method:After methylation,the Supelco SP-2560 capillary column was used as the analysis column,and the samples were detected by temperature program and hydrogen flame ionization detector.Result:The 10 kinds of trans fatty acids in olive oil could be separated well without interference from other components.The detection limit and quantitation limit,precision and recovery rate,durability and specificity of the method were all good.Conclusion:The method is accurate,reliable,simple and suitable for the determination of trans fatty acids in olive oil.

关 键 词：气相色谱法 橄榄油 反式脂肪酸 

分 类 号：O657.71[理学—分析化学] TS225.19[理学—化学]