BTF3L4 Overexpression Mediates APAP-induced Liver Injury in Mouse and Cellular Models  

作　　者：Junchao Lin Aqiang Fan Zhujin Yifu Qibing Xie Liu Hong Wei Zhou 

机构地区：[1]State Key Laboratory of Cancer Biology,National Clinical Research Center for Digestive Diseases and Xijing Hospital of Digestive Diseases,Air Force Military Medical University,Xi’an,Shaanxi,China [2]Department of Digestive Surgery,Xijing Hospital,Air Force Military Medical University,Xi’an,Shaanxi,China

出　　处：《Journal of Clinical and Translational Hepatology》2024年第3期245-256,共12页临床与转化肝病杂志（英文版）

基　　金：funded by Independent Funds of the Key Laboratory(CBSKL2022ZZ34);Xijing Hospital Booster Program(XJZT21CM04).

摘　　要：Background and Aims:Acetaminophen(APAP)-induced liver injury(AILI)has an increasing incidence worldwide.However,the mechanisms contributing to such liver injury are largely unknown and no targeted therapy is currently available.The study aimed to investigate the effect of BTF3L4 overexpression on apoptosis and inflammation regulation in vitro and in vivo.Methods:We performed a proteomic analysis of the AILI model and found basic transcription factor 3 like 4(BTF3L4)was the only outlier transcription factor overexpressed in the AILI model in mice.BTF3L4 overexpression increased the degree of liver injury in the AILI model.Results:BTF3L4 exerts its pathogenic effect by inducing an inflammatory response and damaging mitochondrial function.Increased BTF3L4 expression increases the degree of apoptosis,reactive oxygen species generation,and oxidative stress,which induces cell death and liver injury.The damage of mitochondrial function by BTF3L4 triggers a cascade of events,including reactive oxygen species accumulation and oxidative stress.According to the available AILI data,BTF3L4 expression is positively associated with inflammation and may be a potential biomarker of AILI.Conclusions:Our results suggest that BTF3L4 is a pathogenic factor in AILI and may be a potential diagnostic maker for AILI.

关 键 词：BTF3L4 APAP-induced liver injury Apoptosis Mitochondrial morphology INFLAMMATION 

分 类 号：R575[医药卫生—消化系统]