Clathrin light chains negatively regulate plant immunity by hijacking the autophagy pathway  

作　　者：Hu-Jiao Lan Jie Ran Wen-Xu Wang Lei Zhang Ni-Ni Wu Ya-Ting Zhao Min-Jun Huang Min Ni Fen Liu Ninghui Cheng Paul A.Nakata Jianwei Pan Steven A.Whitham Barbara J.Baker Jian-Zhong Liu 

机构地区：[1]Zhejiang Provincial Key Laboratory of Biotechnology on Specialty Economic Plants,Zhejiang Normal University,Jinhua 321004,China [2]Institute of Plant Genetics and Developmental Biology,College of Chemistry and Life Sciences,Zhejiang Normal University,Jinhua 321004,China [3]College of Life Sciences,Lanzhou University,Lanzhou 730000,China [4]Department of Plant Pathology and Microbiology,Iowa State University,Ames,IA 50011,USA [5]Department of Plant and Microbial Biology,University of California,Berkeley,Berkeley,CA 94720,USA [6]Plant Gene Expression Center,U.S.Department of Agriculture-Agricultural Research Service,Albany,CA 94706,USA [7]Lushan Botanical Garden,Chinese Academy of Sciences,Jiujiang 332000,China [8]U.S.Department of Agriculture-Agricultural Research Service,Children’s Nutrition Research Center,Department of Pediatrics,Baylor College of Medicine,Houston,TX 77030,USA

出　　处：《Plant Communications》2024年第8期172-188,共17页植物通讯（英文）

基　　金：supported by grants from the National Natural Science Foundation of China(32170761,31571423 and 31371401 to J.-Z.L.and 91754104,31820103008,and 31670283 to J.P.);the Iowa State University Plant Sciences Institute and USDA National Institute of Food and Agriculture Hatch project 4308。

摘　　要：The crosstalk between clathrin-mediated endocytosis(CME)and the autophagy pathway has been reported in mammals;however,the interconnection of CME with autophagy has not been established in plants.Here,we report that the Arabidopsis CLATHRIN LIGHT CHAIN(CLC)subunit 2 and 3 double mutant,clc2-1 clc3-1,phenocopies Arabidopsis AUTOPHAGY-RELATED GENE(ATG)mutants in both autoimmunity and nutrient sensitivity.Accordingly,the autophagy pathway is significantly compromised in the clc2-1 clc3-1 mutant.Interestingly,multiple assays demonstrate that CLC2 directly interacts with ATG8h/ATG8i in a domain-specific manner.As expected,both GFP-ATG8h/GFP-ATG8i and CLC2-GFP are subjected to autophagic degradation,and degradation of GFP-ATG8h is significantly reduced in the clc2-1 clc3-1 mutant.Notably,simultaneous knockout of ATG8h and ATG8i by CRISPR-Cas9 results in enhanced resistance against Golovinomyces cichoracearum,supporting the functional relevance of the CLC2-ATG8h/8i interactions.In conclusion,our results reveal a link between the function of CLCs and the autophagy pathway in Arabidopsis.

关 键 词：AUTOPHAGY clathrin light chain immunity clathrin-mediated endocytosis SENESCENCE cell death 

分 类 号：Q94[生物学—植物学]