微生物合成阿魏酸的研究进展  

Advances in Ferulic Acid Production by Engineered Microorganisms

作　　者：李园子 谢琴 廖永红[1] 王凤寰[1] LI Yuanzi;XIE Qin;LIAO Yonghong;WANG Fenghuan(School of Light Industry Science and Engineering,Beijing Technology and Business University,Beijing 100048,China)

机构地区：[1]北京工商大学轻工科学与工程学院,北京100048

出　　处：《中国生物工程杂志》2024年第9期113-125,共13页China Biotechnology

基　　金：国家自然科学基金(32100068);北京市教育委员会科技计划(KM202210011009)资助项目。

摘　　要：阿魏酸是一种来源于植物的酚酸,具有抗氧化、降血脂、抗癌等药理作用,被广泛应用于医药、化妆品和食品行业。从植物中提取和化学合成阿魏酸存在提取效率低、催化成本高、产量低及环境污染严重等问题。微生物合成法具有高效、经济和环保等优势,将成为未来制备阿魏酸的一种趋势。综述了阿魏酸的微生物合成研究进展,讨论了改造底盘菌株、构建异源路径、筛选和优化关键酶、供应和循环辅因子、提高菌株稳定性、细胞共培养、优化发酵条件等提高产量的策略,并就未来的发展趋势提出展望。Ferulic acid is a plant phenolic compound with various pharmacological activities such as antioxidant,blood lipid lowering,and anti-cancer,which has a wide application in the pharmaceutical,cosmetic and food industries.Extraction from plants and chemical synthesis have some problems such as low extraction efficiency,high catalyst cost,low production,and environmental pollution.The biosynthesis of ferulic acid through the construction of microbial cell factories is efficient,economical,and environmentally friendly,and will become a trend for the production of ferulic acid in the future.The synthesis of ferulic acid in engineered microorganisms is summarized,the optimization strategies,such as modification of the host strains,selection and optimization of the key enzymes,supply and recycling of the cofactors,cell co-culture,enhancement of the strain stability,and optimization of the fermentation conditions,to improve the ferulic acid production are discussed,and the future prospects of the ferulic acid biosynthesis are proposed.

关 键 词：阿魏酸 生物合成 代谢工程 微生物 

分 类 号：Q815[生物学—生物工程]