复合诱变筛选提高花生粕中AFB1降解率的菌株  

The Screening of Strains with Higher Degradation Rate of AFB1 in Peanut Meal by Compound Mutagenesis

作　　者：宋静静 王克芬 张辉 张强 张法玲 滕来宾 赵遵明 SONG Jingjing;WANG Kefen;ZHANG Hui;ZHANG Qiang;ZHANG Faling;TENG Laibin;ZHAO Zunming(Shandong Long Kete Enzyme Co.,Ltd,Shandong Province Key Laboratory of Enzyme Preparation Fermentation Technology,Linyi Shandong 276400,China;Yishui County Centre for Animal Disease Control and Prevention,Linyi Shandong 276400,China)

机构地区：[1]山东隆科特酶制剂有限公司,山东省酶制剂发酵技术重点实验室,山东临沂276400 [2]山东省沂水县动物疫病预防控制中心,山东临沂276400

出　　处：《现代畜牧科技》2024年第10期49-52,共4页Modern Animal Husbandry Science & Technology

基　　金：山东省重点研发计划(2022CXGC020801)。

摘　　要：选用产葡萄糖氧化酶(GOD)的黑曲霉菌株H-01为诱变出发菌株,经ARTP-NTG复合诱变后,筛选出1株酶活力提高46%的突变株HAN-40。在酶解条件:温度40℃,料液质量体积比1∶5,GOD添加量5 U/g,酶解时间24 h,突变株HAN-40较原始菌株所产GOD对花生粕中AFB1降解率提高了35%,达到92%。Aspergillus niger strain H-01,which produces glucose oxidase(GOD),was selected as the mutation strain.After the combined mutation of ARTP-NTG,a mutant strain named HAN-40 was selected.The enzyme activity of GOD produced by HAN-40 increased by 46%,which was higher than that of the original strain.Under the enzymolysis conditions:40℃,the mass volume ratio of solid to liquid 1∶5,GOD dosage 5 U/g,the enzymolysis time 24 h,the degradation rate of AFB1 in peanut meal of GOD produced by mutant HAN-40 increased by 35%compared with the original strain.And the degradation rate of AFB1 in peanut meal reached to 92%.

关 键 词：花生粕 黄曲霉毒素 葡萄糖氧化酶 诱变 

分 类 号：S816[农业科学—饲料科学]