Advances in nanoprobes-based immunoassays  

作　　者：Lusi Zhang Bin Huang Jing Jin Yan Li Ning Gu 

机构地区：[1]Jiangsu Key Laboratory for Biomaterials and Devices,School of Biological Science and Medical Engineering,Southeast University,Nanjing,China [2]School of Biomedical Engineering and Informatics,Nanjing Medical University,Nanjing,China [3]Nanjing RealMind Biotech Co.,Ltd.,Nanjing Economic and Technological Development Zone,Nanjing,China [4]Medical School,Nanjing University,Nanjing,China

出　　处：《BMEMat(BioMedical Engineering Materials)》2024年第1期49-75,共27页生物医学工程材料（英文）

基　　金：National Natural Science Foundation of China,Grant/Award Numbers:51832001,61821002;Natural Science Foundation of Jiangsu Province,Grant/Award Number:BK20222002;Nanjing Science and Technology Development Foundation,Grant/Award Number:202205066。

摘　　要：Immunoassay is a powerful technique that uses highly specific antigenantibody interactions to detect biochemical targets such as proteins and toxins.As a diagnostic tool,immunoassay is employed in the screening,diagnosis,and prognosis of diseases,which are crucial for the grasp and control of patient conditions in clinical practice.With the rapid development of nanotechnology,immunoassays based on nanoprobes have attracted more and more attention due to the advantages of high sensitivity,specificity,stability,and versatility.These nanoprobes are nanoscale particles that can act as signal carriers or targeting agents for immunoassays.In this paper,we review the recent advances in various types of nanoprobes for immunoassays,such as colloidal gold,quantum dots,magnetic nanoparticles,nanozymes,aggregation-induced emission,and up-conversion nanoparticles.The effect of the nanoprobe construction and synthesis methods on their detection performance deserves to be studied in depth.We also compare their detection ranges and limits in different immunoassay methods,such as lateral flow immunoassays,fluorescent immunoassays,and surface-enhanced Raman scattering immunoassays.Moreover,we discuss the benefits and challenges of nanoprobes in immunoassays and provide insights into their future development.This study aims to offer a comprehensive and critical perspective on the role of nanoprobes in the field of immunoassays.

关 键 词：detection range IMMUNOASSAY limit of detection NANOPROBES 

分 类 号：R318[医药卫生—生物医学工程] TB383[医药卫生—基础医学]