The involvement of Slc7a11/xCT related iron accumulation on acupuncture intervention in 6-OHDA-induced Parkinson rat  

作　　者：Lihong Li Wei Jin Jun Lu Zitong Xu Zhaoshun Lv Jianquan Yin Wenjie Cong 

机构地区：[1]Acupuncture Department,The Second Affiliated Hospital of Zhejiang Chinese Medical University(Xinhua Hospital of Zhejiang Province),Hangzhou 310005,Zhejiang,China [2]The First School of Clinical Medicine,Zhejiang Chinese Medical University,Hangzhou 310053,Zhejiang,China [3]Urological Department,Zitong Hospital of Traditional Chinese Medicine,Mianyang 622150,Sichuan,China [4]The Second School of Clinical Medicine,Zhejiang Chinese Medical University,Hangzhou 310053,Zhejiang,China [5]Acupuncture Department,Zhejiang Provincial People’s Hospital,Hangzhou 310004,Zhejiang,China [6]Acupuncture and Physiotherapy Department,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou 325015,Zhejiang,China

出　　处：《Clinical Traditional Medicine and Pharmacology》2024年第4期34-43,共10页临床传统医学和药理学（英文）

基　　金：supported by Natural Science Foundation of China(No.82104982);Zhejiang Funding of Traditional Chinese Medicine(GZY-ZJ-KJ-24016,GZY-ZJ-KJ-23017);Natural Science Foundation of Zhejiang province,China(No.LGD19H090005).

摘　　要：Background:Acupuncture plays a critical role for the treatment of patients with Parkinson’s disease(PD),which is characterized by ferroptosis of dopaminergic neurons.It has been found that acupuncture can reduce iron accumulation.However,the mechanism of acupuncture therapy against PD by inhibiting ferroptosis remains unclear.Objective:To investigate the potential mechanism of acupuncture on alleviating ferroptosis in 6-OHDA-induced PD rats.Methods:Male Sprague-Dawley rats were randomly divided into sham group,acupuncture group,and PD model group,with the PD model induced by 6-OHDA.The motor function of the rats was tested by the rotation test and suspension test,respectively.Immunohistochemistry and iron staining were performed to analyze nerve damage,and the effect of acupuncture on the accumulation of iron in the substantia nigra(SN).RNA-seq analysis in the SN of rats were performed to discover ferroptosis-related genes.Differentially expressed genes(DEGs)resulted from the acupuncture treatment were identified and were used for Gene Ontology(GO)function,Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analyses.A protein–protein interaction(PPI)network was constructed,and alternative splicing(AS)analysis of the transcriptome was performed.Western blot was used to detect the protein level change of Slc7a11/xCT.Results:Rotation test and suspension test indicated a positive effect of acupuncture on body weight gain and limb function.Perl’s iron staining and ELISA test detected 6-OHDA induced iron accumulation in the SN and nerve damage,which was mitigated by acupuncture treatment.Acupuncture treatment brought about the differential expression of a total of 1088 genes in the SN of rats.GO and KEGG enrichment revealed that such DEGs were mainly associated with several key biological process(BP),cellular component(CC)and molecular function(MF)terms.These terms included collagen fibril organization,extracellular matrix organization,collagen trimer,extracellular matrix components,chemokine receptor

关 键 词：Parkinson’s disease RNA-SEQ Slc7a11/xCT ACUPUNCTURE 

分 类 号：R742.5[医药卫生—神经病学与精神病学]