Astragalus polysaccharide orβ-glucan combined with inactivated vaccine markedly prevent CyHV-2 infection in Carassius auratus gibelio  

作　　者：Xingchen Huo Yiyi Yan Jiao Chang Jianguo Su 

机构地区：[1]Department of Aquatic Animal Medicine,College of Fisheries,Huazhong Agricultural University,Wuhan,430070,China [2]Laboratory for Marine Biology and Biotechnology,Pilot National Laboratory for Marine Science and Technology,Qingdao,266237,China [3]Hubei Hongshan Laboratory,Engineering Research Center of Green Development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt,Ministry of Education,Wuhan,430070,China

出　　处：《Aquaculture and Fisheries》2024年第5期763-775,共13页渔业学报（英文）

基　　金：supported by Fundamental Research Funds for the Central Universities (2662021SCPY006);National Key Research and Development Program of China (2018YFD0900504).

摘　　要：Herpesviral hematopoietic necrosis disease caused by cyprinid herpesvirus 2(CyHV-2)results in huge economic losses for the gibel carp(Carassius auratus gibelio)aquaculture industry.A vaccination strategy is a feasible method to prevent CyHV-2 infection and the resulting economic losses.In this study,inactivators(includingβ-propiolactone,formaldehyde and binary ethylenimine)were used to prepare an inactivated vaccine.The optimal inactivated CyHV-2 vaccine(0.2%β-propiolactone inactivates CyHV-2 for 48 h)mixed withβ-glucan,anisodamine,chitosan,and astragalus polysaccharide(APS)adjuvants were injected into gibel carp.Theβ-glucan and APS combined with inactivated vaccine significantly improved the relative immune protection rate of gibel carp against CyHV-2 infection.The highest specific antibody levels inβ-glucan and APS groups were found in serum by ELISA assay and antibody neutralization titer.Furthermore,adjuvant addition groups produced better results for lysozyme,total superoxide dismutase,and complement C3 in serum.β-glucan and APS combined with vaccine treatment effectively enhanced mRNA expressions of important immune genes(IL-1β,IgM,IL-2 and IFN-γ2).Minimal tissue lesions(gill,spleen,and head kidney)and virus replication were seen in theβ-glucan and APS groups by histopathological examination and expression analysis of CyHV-2 TK gene.These results indicated that the combination of inactivated vaccine and adjuvantβ-glucan or APS is an effective method against CyHV-2 infection and provided a case study reference for prevention of fish viral diseases using inactivated vaccines.

关 键 词：Carassius auratus gibelio Cyprinid herpesvirus 2(CyHV-2) Β-GLUCAN ADJUVANT Astragalus polysaccharide(APS) VACCINE 

分 类 号：S917[农业科学—水产科学]