Hypoxia activates autophagy by Akt/FoxO1 pathway in fish cells  

作　　者：Kang Chen Linlin Shi Hong Liu Huanling Wang 

机构地区：[1]Key Lab of Freshwater Animal Breeding,Key Laboratory of Agricultural Animal Genetics,Breeding and Reproduction,Ministry of Education,College of Fisheries,Huazhong Agricultural University,Wuhan,430070,China [2]Engineering Research Center of Green Development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt,Ministry of Education,Wuhan,430070,China

出　　处：《Aquaculture and Fisheries》2024年第4期557-565,共9页渔业学报（英文）

基　　金：supported by Natural Science Foundation of China (31872542,32072965);Fundamental Research Funds for the Central Universities (2662022SCYJ005);China Agriculture Research System of MOF and MARA (No.CARS-45-01).

摘　　要：Hypoxia,a common environmental condition,can affect cell survival and physiological function by triggering oxidative stress.Akt/FoxO pathway has been proven to play a non-negligible role in the regulation of autophagy.However,the role of Akt/FoxO pathway in hypoxia-induced autophagy is unclear in fish.Therefore,in this study,grass carp hepatocyte cells(L8824)were treated by CoCl2 to simulate hypoxia,and the results showed that CoCl2 can increase the expression of Hif-1αprotein at different concentrations or different treatment time.Further study found that hypoxia increased intracellular reactive oxygen species(ROS)level,and the expression of autophagy-related genes(LC3-II,pink1,beclin-1 and p62)and foxO1a/1b.The mitochondrial membrane potential(Δψm)was also depolarized,and autophagosomes were intriguingly detected by transmission electron microscope(TEM)after the treatment of hypoxia.Moreover,hypoxia inhibited Akt phosphorylation,while PI3K/Akt pathway inhibitor,LY294002 significantly up-regulated the expression of foxO1a/1b and autophagy-related genes.Additionally,silencing foxo1b also resulted in down-regulation of autophagy-related genes.It was demonstrated that hypoxia induced autophagy via Akt/FoxO1 pathway.These results will provide a new light on further understanding the role of Akt/FoxO pathway in the response to hypoxia in fish.

关 键 词：HYPOXIA AUTOPHAGY Fish cells AKT FOXO 

分 类 号：S917[农业科学—水产科学]