马铃薯疮痂病生防链霉菌菌株PBSH9发酵条件优化  

Optimization of Fermentation Conditions for the Biocontrol Streptomyces Strain PBSH9 against Potato Scab Disease

作　　者：宋亚迪 郭婷[1,2] 修志君 王丽玮 杨春芳 王淑华 张笑宇 Song Yadi;Guo Ting;Xiu Zhijun;Wang Liwei;Yang Chunfang;Wang Shuhua;Zhang Xiaoyu(College of Horticulture and Plant Protection,Inner Mongolia Agricultural University,Hohhot 010020,Inner Mongolia,China;Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences,Hohhot 010031,Inner Mongolia,China;Aohan Banner Agriculture and Animal Husbandry Bureau,Chifeng 024300,Inner Mongolia,China)

机构地区：[1]内蒙古农业大学园艺与植物保护学院,内蒙古呼和浩特010020 [2]内蒙古自治区农牧业科学院,内蒙古呼和浩特010031 [3]敖汉旗农牧局,内蒙古赤峰024300

出　　处：《作物杂志》2024年第5期255-262,共8页Crops

基　　金：内蒙古自然科学基金(2021MS03053);内蒙古自治区科技计划项目(2022YFYZ0008);国家自然科学基金(32260700);内蒙古自治区特色品种育种公关联合项目(YZ2023006)。

摘　　要：以发酵液菌量为筛选指标,采用单因素方法筛选菌株PBSH9的最佳碳源、氮源、接种量、pH、温度及培养时间,以优化发酵培养条件。结果表明,小麦麸皮最有利于菌株PBSH9的生长,将小麦麸皮以1%浓度替代高氏1号培养基的可溶性淀粉时,菌株PBSH9发酵液菌落数为1.04×10^(11)CFU/mL,抑菌率为60.3%;豆粕饼粉最有利于菌株PBSH9的生长,以0.5%浓度取代高氏1号培养基的硝酸钾时,菌落数及抑菌率最大,分别为1.24×10^(11)CFU/mL和55.6%;将浓度为1×10^(8)CFU/mL种子液以4%比例接种到高氏1号培养基中,发酵液菌量和抑菌率最大,菌落数为1.45×10^(11)CFU/mL,抑菌率为71.9%;菌株PBSH9 pH 6.0~7.0抑菌率最高,分别为66.3%和65.8%;28℃时发酵液菌落数和抑菌率最大,菌落数为1.02×10^(12)CFU/mL,抑菌率为69.1%;在优化发酵培养基中培养6 d时,菌量最大,抑菌率(71.9%)最高。Using the fermentation broth bacterial quantity as the screening index,the single-factor method was used to screen the best carbon source,nitrogen source,inoculum amount,pH,temperature,and incubation duration for strain PBSH9.The findings indicated that the best material for strain PBSH9 development was wheat bran.When wheat bran was used to replace soluble starch in Gaoshi 1 medium with a concentration of 1%,the bacterial colony amount of strain PBSH9 in the fermentation broth was 104.3×10^(9) CFU/mL,and the antibacterial rate was 60.3%.Soybean meal powder is most beneficial for the growth of strain PBSH9,When replacing potassium nitrate in Gaoshi 1 culture medium with 0.5%concentration of soybean meal powder,the maximum number of colonies and antibacterial rate were 123.7×10^(9) CFU/mL and 55.6%,respectively.Inoculating a seed solution with a concentration of 1×10^(8) CFU/mL by 4%ratio into Gaoshi 1 culture medium,the fermentation broth had the highest bacterial amount and antibacterial rate,with a colony amount of 144.6×10^(9) CFU/mL and an antibacterial rate of 71.9%;strain PBSH9 in pH 6.0-7.0 had the strongest bacterial inhibition activity,66.3% and 65.8%,respectively;the fermentation broth had the largest colony size and bacterial inhibition rate at 28℃,with a colony size of 101.6×10^(10) CFU/mL and 69.1%inhibition rate.When cultured in optimized fermentation medium for six days,the maximum bacterial amount and antibacterial rate(71.9%)were achieved.

关 键 词：马铃薯 马铃薯疮痂病 链霉菌 生防菌 发酵条件 

分 类 号：S435.32[农业科学—农业昆虫与害虫防治] S476[农业科学—植物保护]