水洗对稀酸蒸汽爆破玉米秸秆酶水解的影响  

Eff ect of Water Washing on the Enzymatic Hydrolysis of Corn Stover by Dilute Acid Steam Explosion

作　　者：梁井瑞 杜健 高小涵 张超楠 杨曼玉 邓增芹 Liang Jingrui;Du Jian;Gao Xiaohan;Zhang Chaonan;Yang Manyu;Deng Zengqin(College of Food Science and Pharmaceutical Engineering,Zaozhuang University,Shandong,277160)

机构地区：[1]枣庄学院食品科学与制药工程学院,山东277160

出　　处：《当代化工研究》2024年第19期82-84,共3页Modern Chemical Research

基　　金：国家自然科学基金“嗜热子囊菌TaAA9A关键氨基酸介导的结晶纤维素结合与降解机制”(项目编号:32201513);山东省自然科学基金“真菌裂解多糖单加氧酶降解不同结晶类型纤维素的关键结构位点解析及理性改造”(项目编号:ZR2020QC164);枣庄市学院青檀学者人才工程项目(2020批次);山东省高等学校青年创新计划团队(2023KJ282)。

摘　　要：蒸汽爆破是木质纤维素综合利用的常用预处理方法,但常会产生一些抑制物抑制酶水解。以蒸汽爆破玉米秸秆为研究对象,探究水洗处理对木质纤维素酶水解的影响。发现水洗后蒸汽爆破玉米秸秆酶水解的效率提升超过18%,远高于其他碱处理脱毒手段。通过分析底物亲和力、酶活力和形态观察等,解析水洗促进蒸汽爆破玉米秸秆酶水解的机制。结果发现,经过水洗后的蒸汽爆破玉米秸秆的底物与酶亲和力提高了18.8%(Km值从7.84降低至6.60)、内切酶与外切酶活力显著提高,纤维素形态均发生了利于酶水解的改变。Steam explosion is a commonly used pretreatment method for the comprehensive utilization of lignocellulose,but it often produces some inhibitors that inhibit enzyme hydrolysis.Taking steam explosion of corn straw as the research object,this study investigates the effect of water washing treatment on the hydrolysis of lignocellulosic enzymes.It was found that the efficiency of steam explosion corn straw enzymatic hydrolysis after washing increased by more than 18%,which is much higher than other alkaline treatment methods for detoxification.By analyzing substrate af-finity,enzyme activity,and morphological observation,we aim to elucidate the mechanism of water washing promoting enzymatic hydrolysis of steam exploded corn stover.The results showed that the substrate and enzyme affinity of steam exploded corn stover after washing increased by 18.8%(Km value decreased from 7.84 to 6.60),and the activities of endonucleases and exonucleases were significantly increased.The cellulose morphology un-derwent changes that were conducive to enzymatic hydrolysis.

关 键 词：蒸汽爆破 木质纤维素 水洗 纤维素酶 

分 类 号：TQ35[化学工程]