蛋源叶酸对脂多糖诱导孕鼠氧化应激健康效应及胎鼠神经管缺陷的保护作用  

Egg-Derived Folic Acid Protects against Lipopolysaccharide-Induced Oxidative Stress in Pregnant Mice and Neural Tube Defects in Fetal Mice

作　　者：李小萌 王玉林 孙浩洋 徐李根 黄茜[1] 盛龙[1] 蔡朝霞[1] LI Xiaomeng;WANG Yulin;SUN Haoyang;XU Ligen;HUANG Xi;SHENG Long;CAI Zhaoxia(Key Laboratory of Egg Processing,Ministry of Agriculture and Rural Affairs,College of Food Science and Technology,Huazhong Agricultural University,Wuhan 430070,China)

机构地区：[1]农业农村部蛋品加工重点实验室,华中农业大学食品科学技术学院,湖北武汉430070

出　　处：《食品科学》2024年第21期48-57,共10页Food Science

基　　金：中央高校基本科研业务费专项(2662024SPPY002)。

摘　　要：比较化学合成叶酸和天然蛋黄来源叶酸对由脂多糖诱导的孕鼠健康效应及胎鼠神经管缺陷的保护作用。通过观察胎鼠的生长发育状况和外观,测定孕鼠体内的叶酸消化吸收、氧化还原内稳态,以及胎盘健康状况,探讨不同来源叶酸的消化吸收差异和对孕鼠氧化应激水平的影响。通过评估胎鼠脑部神经管发育状态、神经管相关蛋白表达情况、抗氧化水平和炎症因子表达量解析叶酸来源对预防胎鼠神经管缺陷和调节体内炎症水平的潜力。研究发现,不同来源的叶酸均能有效改善孕鼠胎盘的健康状况,其中蛋源叶酸更有利于减少脂多糖造成的损伤,并且呈现出剂量依赖关系。蛋源叶酸对胎鼠神经管细胞的发育呈正反馈调节,能够平衡胎鼠脑部的超氧化物歧化酶、过氧化氢酶、谷胱甘肽酶的水平,使胎鼠体内的IL-6、IL-1β、MCP-1、iNOS和TNF-α mRNA的相对表达量处于健康平衡的状态。高剂量蛋源叶酸组中与促进神经管发育相关的蛋白质神经细胞黏附分子1表达显著增加,表现出良好的预防神经管缺陷的潜力以及抗炎效果。这些结果突出了蛋源叶酸在预防神经管缺陷和调节体内炎症水平方面的重要性,为食物来源叶酸健康功能提供了新的理论和实践依据。This study compared the effects of chemically synthesized folic acid and natural folic acid derived from duck egg yolk on health outcomes in pregnant mice induced by lipopolysaccharide(LPS)and their protective effects against neural tube defects in fetal mice.By observing the growth and appearance of fetal mice as well as assessing folate digestion and absorption in pregnant mice,redox homeostasis,and placental health,differences in the absorption of folates from different sources and their effects on oxidative stress levels in pregnant mice were explored.Evaluation of neural tube development in fetal mice,neural tube-related protein expression,antioxidant levels and inflammatory cytokine expression was conducted to analyze the potential of folate sources in preventing fetal neural tube defects and regulating inflammation levels in the body.The results demonstrated that both synthetic and egg-derived folic acids effectively improved the placental health of pregnant mice,with the latter being more effective in reducing LPS-induced damage in a dose-dependent manner.Egg-derived folate regulated the development of neural tube cells in fetal mice in a positive feedback manner,balanced the levels of superoxide dismutase,catalase,and glutathione peroxidase in the brain of fetal mice,and maintained the relative mRNA expression levels of IL-6,IL-1β,MCP-1,iNOS,and TNF-αin a healthy balance.High-dose egg-derived folate significantly increased the expression of neural cell adhesion molecule 1(NCAM1),a protein associated with promoting neural tube development,demonstrating good potential for preventing neural tube defects and inhibit inflammation.These results underscore the importance of egg-derived folate for preventing neural tube defects and regulating inflammation in the body,providing a new theoretical and practical basis for developing the health benefits of food-derived folate.

关 键 词：蛋源叶酸 合成叶酸 神经管缺陷 炎症 氧化应激 

分 类 号：S873[农业科学—畜牧兽医] TS201.4[轻工技术与工程—食品科学]