鸡蛋清蛋白质糖肽的结构鉴定  

Structural Identification of Glycopeptides Derived from Chicken Egg White Proteins

作　　者：蔡芝鸿 何洪 王梅[1] 王郁婷 李述刚 王金秋[1] 耿放[1] CAI Zhihong;HE Hong;WANG Mei;WANG Yuting;LI Shugang;WANG Jinqiu;GENG Fang(School of Food and Biological Engineering,Chengdu University,Chengdu 610106,China;Clinical Medical College,Chengdu University,Chengdu 610106,China;School of Food and Biological Engineering,Hefei University of Technology,Hefei 230601,China)

机构地区：[1]成都大学食品与生物工程学院,四川成都610106 [2]成都大学临床医学院,四川成都610106 [3]合肥工业大学食品与生物工程学院,安徽合肥230601

出　　处：《食品科学》2024年第21期58-67,共10页Food Science

基　　金：四川省自然科学基金面上项目(2023NSFSC0183,2024NSFSC0366);现代农业产业技术体系建设专项(CARS-40-K25)。

摘　　要：鸡蛋清中主要蛋白质均为糖蛋白,经胃肠消化后将在消化道内产生大量糖肽,这些糖肽的结构需要被鉴定和明确。本研究采用胰蛋白酶将鸡蛋清消化,然后从水解物中通过亲水相互作用层析富集N-糖肽、以Retain AX层析结合AminoLink树脂吸附富集O-糖肽,运用液相色谱-质谱联用技术对糖肽结构进行解析,并借助生物信息学分析探究蛋清糖蛋白对肠道的潜在作用。经质谱解析,共鉴定到来自于49种N-糖蛋白的1720个N-糖肽结构,以及来自29种O-糖蛋白的565个O-糖肽结构。其中,卵黏蛋白糖肽结构最为多样,共有490个N-糖肽结构和216个O-糖肽结构。京都基因与基因组百科全书通路富集分析发现,含唾液酸修饰的N-糖蛋白主要涉及白细胞介素-17信号通路和铁死亡,而含唾液酸修饰的O-糖蛋白主要涉及胆固醇代谢、维生素消化吸收、低氧诱导因子-1信号通路和肾素-血管紧张素系统。本研究系统解析了鸡蛋清蛋白质的糖肽结构,为后续探究蛋清糖肽对肠道的潜在作用提供了重要的结构信息。The primary proteins in chicken egg white are glycoproteins,which generate substantial glycopeptides in the digestive tract following gastrointestinal digestion.The structures of the glycopeptides have yet to be identified and elucidated.In this study,chicken egg white was digested by trypsin,and N-glycopeptides were enriched from the hydrolysate by hydrophilic interaction chromatography and O-glycopeptides by retain AX chromatography combined with AminoLink resin adsorption.The structures of these glycopeptides were analyzed by liquid chromatography-mass spectrometry(LC-MS),and their potential intestinal effects were explored by bioinformatics analysis.Mass spectrometry analysis identified 1720 N-glycopeptides from 49 N-glycoproteins and 565 O-glycopeptides from 29 O-glycoproteins.Glycopeptides derived from ovomucin were the most diverse,including 490 N-glycopeptides and 216 O-glycopeptides.Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis revealed that sialylated N-glycoproteins were mainly involved in interleukin-17 signaling pathway and ferroptosis,while sialylated O-glycoproteins were mainly involved in cholesterol metabolism,vitamin digestion and absorption,the hypoxia-inducible factor-1 signaling pathway and the renin-angiotensin system.This study provides crucial structural information for further research on the potential effects of egg white glycopeptides on the intestinal tract.

关 键 词：鸡蛋清蛋白质 N-糖基化 O-糖基化 糖蛋白质组学 

分 类 号：TS253.2[轻工技术与工程—农产品加工及贮藏工程]