史氏鲟鱼精肽的制备、体外抗炎活性评价和结构表征  

Preparation,in Vitro Anti-inflammatory Activity and Structural Characterization of Acipenser schrenckii Milt Peptide

作　　者：刘文博 赵青 李海蓝[1] 廖涛[1,2] 鉏晓艳 LIU Wenbo;ZHAO Qing;LI Hailan;LIAO Tao;ZU Xiaoyan(Key Laboratory of Cold Chain Logistics Technology for Agro-product(Ministry of Agriculture and Rural Affairs),Institute of Agro-products Processing and Nuclear Agricultural Technology,Hubei Academy of Agricultural Sciences,Wuhan 430064,China;School of Chemistry and Environmental Engineering,Wuhan Institute of Technology,Wuhan 430205,China;School of Life and Health Sciences,Hubei University of Technology,Wuhan 430068,China)

机构地区：[1]湖北省农业科学院农产品加工与核农技术研究所,农业农村部农产品冷链物流技术重点实验室,湖北武汉430064 [2]武汉工程大学化学与环境工程学院,湖北武汉430205 [3]湖北工业大学生命科学与健康工程学院,湖北武汉430068

出　　处：《食品科学》2024年第21期112-120,共9页Food Science

基　　金：湖北省技术创新计划重点研发专项(2022BBA0011)。

摘　　要：旨在研究史氏鲟(Acipenser schrenckii)鱼精肽(sturgeon milt peptide,SMP)的抗炎潜力及其分子特性。首先对鲟鱼精蛋白进行提取、酶解、纯化得到SMP,评估了其对一氧化氮(NO)和牛血清白蛋白变性的抑制效果。然后利用现代波谱技术测定其分子质量、二级结构、晶体结构、质子峰分布和氨基酸序列等,并基于此预测氨基酸序列的抗炎活性。结果显示,在10 mg/mL质量浓度下,SMP对NO和牛血清蛋白变性的抑制率分别为45.33%和60.34%,显著高于鲟鱼精蛋白(P<0.05)。SMP中碱性和疏水性氨基酸含量分别占37.45%和36.77%,其中精氨酸占比最高(16.28%)。SMP分子质量集中于206~1040 Da之间,主要呈现不规则的无定形结构,水溶液状态下暴露更多的疏水性基团。SMP中含有较多的短肽,包括MPY、YWH、YPY和VPPL等,这些肽序列具有抗炎特性。综上,SMP具有潜在的抗炎活性,是一种有前途的天然抗炎肽来源,具有进一步开发和应用的潜力。The aim of this study was to investigate the anti-inflammatory potential and molecular properties of sturgeon(Acipenser schrenckii)milt peptide(SMP).SMP was obtained from alkali-extracted protein from sturgeon milt by enzymatic hydrolysis and purification,and its inhibitory effect on nitric oxide(NO)and bovine serum albumin(BSA)denaturation was evaluated.Then,the molecular mass,secondary structure,crystalline structure,proton peak distribution and amino acid sequence were determined by various spectroscopies,and based on the obtained results,the anti-inflammatory activity of amino acid sequences was predicted.The results showed that at a concentration of 10 mg/mL,SMP inhibited NO and BSA degradation by 45.33%and 60.34%,respectively,which was significantly more effective than sturgeon milt protein(P<0.05).Basic and hydrophobic amino acids accounted for 37.45%and 36.77%the total amino acids of SMP,respectively,with arginine accounting for the highest proportion(16.28%).The molecular masses of SMP were concentrated in the range of 206–1040 Da,which mainly showed an irregular amorphous structure and exposed more hydrophobic groups in aqueous solution.SMP contained many short peptides,including MPY,YWH,YPY and VPPL,and these peptide sequences had anti-inflammatory properties.In summary,SMP has potential anti-inflammatory activity and possesses the potential for further development and application as a promising source of natural anti-inflammatory peptides.

关 键 词：鲟鱼精肽 抗炎活性 结构表征 氨基酸序列 

分 类 号：TS254.9[轻工技术与工程—水产品加工及贮藏工程]