氯化钙采前喷施对西梅采后果实活性氧代谢的调节作用  

Regulation of postharvest fruit reactive oxygen metabolism by preharvest spraying of calcium chloride on prunes

作　　者：田全明 祁华梅 殷贝贝 魏佳 张政 王曼 邢世均 袁宇尧 吴斌 TIAN Quanming;QI Huamei;YAN Beibei;WEI Jia;ZHANG Zheng;WANG Man;XIN Shijun;YUAN Yuyao;WU Bin(Lingnan Normal University,Zhanjiang Guangdong 524048,China;College of Horticulture,Xinjiang Agricultural University,Urumqi 830052,China;School of Life Science and Technology,Xinjiang University,Urumqi 830046,China;Research Institute of Farm Product Storage and Processing,Xinjiang Academy of Agricultural Sciences,Urumqi 830091,China;Key Laboratory Of Agricultural Products Processing And Preservation,Urumqi 830091,China)

机构地区：[1]岭南师范学院,广东湛江524048 [2]新疆农业大学园艺学院,乌鲁木齐830052 [3]新疆大学生命科学与技术学院,乌鲁木齐830046 [4]新疆农业科学院农产品贮藏加工研究所,乌鲁木齐830091 [5]新疆农产品加工与保鲜重点实验室,乌鲁木齐830091

出　　处：《新疆农业科学》2024年第8期1937-1946,共10页Xinjiang Agricultural Sciences

基　　金：科技创新领军人才项目-高层次领军人才(2022TSYCLJ0040);新疆维吾尔自治区重点研发计划(2022B02026-1)。

摘　　要：【目的】研究CaCl_(2)(Calcium chloride)采前喷施对西梅果实采后衰老的抑制作用。【方法】以新疆喀什地区伽师县法兰西西梅为试材,采用2%CaCl_(2)在果实的膨大期、转色期和采收期喷施,测定活性氧代谢相关酶活性,分析果实衰老与活性代谢之间的关系。【结果】采前CaCl_(2)处理可延缓西梅采后果实失重率、总酚和类黄酮的下降,抑制西梅采后果实中丙二醛含量的升高、加快清除超氧阴离子、过氧化氢。采前CaCl_(2)处理显著提高过氧化物酶(peroxidase,POD)、超氧化物歧化酶(superoxide dismutase,SOD)、过氧化氢酶(catalase,CAT)、抗坏血酸过氧化物酶(ascorbate peroxidase,APX)、谷胱甘肽还原酶(Glutathione reductase,GR)的活性,从而延缓果实采后失重率的下降。贮藏至第21 d,CaCl_(2)处理果实的失重率提高了67.05%,POD、SOD、CAT、APX、GR的活性分别提高了30.21%、15.87%、48.41%、57.46%和50.49%。【结论】CaCl_(2)采前处理减少了活性氧的积累,减轻了西梅果实的氧化损伤,维持细胞膜的完整性,进而延缓了西梅果实的衰老。【Objective】To investigate the inhibitory effect of pre-harvest spraying of CaCl_(2)(Calcium chloride)on post-harvest senescence of prune fruits.【Methods】French prunes from Gashi County,Kashgar Prefecture,Xinjiang,as the test material for the study.The relationship between fruit senescence and active metabolism was analyzed by measuring the activities of enzymes related to active oxygen metabolism using 2%CaCl_(2)sprayed at the expansion,colour change and harvesting stages of the fruit.【Results】The results showed that the pre-harvest CaCl_(2)treatment delayed the decrease of post-harvest fruit weight loss,total phenols and flavonoids,and inhibited the increase of malondialdehyde and accelerated the scavenging of superoxide anion and hydrogen peroxide in the post-harvest fruits of prunes.Pre-harvest CaCl_(2)treatment significantly increased the activity of Peroxidase(POD),Superoxide Dismutase(SOD),catalase(CAT),Ascorbate Peroxidase(APX)and Glutathione Reductase(GR),thereby delaying the decline in post-harvest fruit weight loss.After 21 days storage,the weight loss rate of fruits treated with CaCl_(2)increased by 67.05%.The acitivies of POD、SOD、CAT、APX、GR increased by 30.21%、15.87%、57.46%and 50.49%respectively.【Conclusion】Therefore,CaCl_(2)pre-harvest treatment reduces the accumulation of reactive oxygen species,mitigates oxidative damage in prune fruit,maintains the integrity of cell membranes thus delaying the ageing of prune fruit.

关 键 词：氯化钙 西梅 活性氧代谢 衰老 

分 类 号：TS202.1[轻工技术与工程—食品科学]