臭参粗多糖热水浸提工艺及抗氧化性研究  

Study on the extraction process and antioxidant properties of Codonopsis Pilosula polysaccharide

作　　者：赵红艳 张鸭关 陈德瑞 ZHAO Hong-yan;ZHANG Ya-guan;CHEN De-rui(School of Chemistry and EnvironmentScience,Qujing Normal University,Qujing 655011,China)

机构地区：[1]曲靖师范学院化学与环境科学学院,云南曲靖655011

出　　处：《化学工程师》2024年第10期90-94,共5页Chemical Engineer

基　　金：云南省教育厅项目(2022J0816)。

摘　　要：为研究臭参多糖提取工艺,以3年生臭参的地下部分为原料,设计提取温度、提取时间和料液比进行单因素实验,在此基础上,进行三因素三水平正交实验优化得到最佳提取工艺,并对提取的粗多糖进行了抗氧化性研究。结果表明,臭参多糖提取的最佳条件为浸提温度为90℃、浸提时间为40min、料液比为1∶60(g∶mL),在此条件下多糖得率为24.61%。在一定浓度范围内,臭参多糖溶液的总还原能力随浓度的增大而增大,总还原能力与浓度之间表现出较好的相关性。臭参多糖与VC(维生素C)对DPPH·(1,1-二苯基-2-苦基肼自由基)清除率在浓度为12mg·mL^(-1)时相近,多糖和VC清除率分别达到96.39%和100%。臭参多糖的提取率较高,抗氧化活性显著,为臭参多糖的开发利用提供依据。To study the optimum conditions of extraction of polysaccharides from Codonopsis Pilosula,a single factor experiment was conducted using the underground part of three-year-old Codonopsis Pilosula as the raw material,and extraction temperature,time and liquid-solid ratio were designed.Based on this,a three factor three-level orthogonal expriment was conducted to optimize the extraction process,and the antioxidant activities of the polysaccharides were studied.The results showed that the optimum extraction conditions were obtained as follows:extraction temperature of 90℃,extraction time of 40min,solid-liquid ratio of 1∶60(g∶mL).Under these conditions,the extraction yields of Codonopsis Pilosula polysaccharides were 24.61%.In a certain concentration range,the scavenging ability increased with the increase of the polysaccharides concentration and showed a good correlation between the total scavenging ability and the concentration.The scavenged ability of the polysaccharides and VC on DPPH·were similar at 12mg·mL^(-1)and the scavenging rates of polysaecharides and VC were 96.39%and 100%,respectively.The extraction rate of stinky ginseng polysacharides was hign and antioxidant activity was significant,which provides a basis for the development and utilization of the polysaccharide in the later period.

关 键 词：臭参 正交实验 多糖 抗氧化性 热水浸提 

分 类 号：Q949.95[生物学—植物学]