气相色谱法测定调制羊奶粉中37种脂肪酸  

Determination of 37 Fatty Acids in Formula Sheep Milk Powder by Gas Chromatography

作　　者：张清 曾令英 周娇 吴芸芸 李上 李南燕 兰姝姝 王贵敏 陈婷 房翠兰[1] ZHANG Qing;ZENG Lingying;ZHOU Jiao;WU Yunyun;LI Shang;LI Nanyan;LAN Shushu;WANG Guimin;CHEN Ting;FANG Cuilan(Chongqing Jiulongpo District Center for Disease Control and Prevention,Chongqing 400039,China)

机构地区：[1]重庆市九龙坡区疾病预防控制中心,重庆400039

出　　处：《食品与发酵科技》2024年第5期121-126,共6页Food and Fermentation Science & Technology

基　　金：重庆市公共卫生重点专科(学科)建设经费资助(YWBF2022072);重庆市九龙坡区公共卫生重点实验室建设经费资助(JYBF2023063);JLPCDC-ZL2023107;2023-02-034-Z;2023QNXM022。

摘　　要：建立了气相色谱法(Gas Chromatography,GC)同时测定调制羊奶粉中37种脂肪酸的分析方法。样品经氨水水解,用乙醚-石油醚混合液(1∶1,v/v)提取,提取物经2%氢氧化钠-甲醇溶液皂化,15%三氟化硼-甲醇溶液甲酯化,正庚烷提取后用GC测定。37种脂肪酸在各自的质量浓度范围内线性关系良好(R2>0.995),方法检出限为0.002~0.006 g/100 g,方法定量限为0.01~0.03 g/100 g。质控样的平均加标回收率为83.3%~108.5%,相对标准偏差为0.9%~5.6%(n=6)。该方法操作简便、准确可靠,用于测定市场购买的16组调制羊奶粉,结果发现市售调制羊奶粉中脂肪酸的组成及含量存在差异。A gas chromatography(GC)method was established to determine the content of 37 fatty acids in formula sheep milk powder.Sheep milk powder was hydrolyzed by ammonia water and extracted with a mixture of ether and petroleum ether(1∶1,v/v).The extract was saponified with 2%sodium hydroxide-methanol solution,methylated with a 15%boron trifluoride-methanol solution,and extracted by heptane for GC determination.37 fatty acids had good linear relationships in their respective mass concentration range(R2>0.995).The detection limit of the method was 0.002~0.006 g/100 g,and the quantification limit of the method was 0.01~0.03 g/100 g.The average spiked recovery rate of quality control samples was 83.3%~108.5%,with a relative standard deviation of 0.9%~5.6%(n=6).This method is easy to operate,accurate and reliable.By applying this method to measure 16 batches of formula sheep milk powder purchased in the market,it was found that there were differences in the composition and content of fatty acids in formula sheep milk powder.

关 键 词：气相色谱法 调制羊奶粉 脂肪酸 脂肪酸甲酯 

分 类 号：TS207.3[轻工技术与工程—食品科学]