白屈菜红碱抗耐甲氧西林金黄色葡萄球菌作用机制研究  

The Mechanism of Chelerythrine against Methicillin-resistant Staphylococcus aureus

作　　者：苑庆欣 刘阔 包旭华 高东阳 李鹤[1] 宋军 周志新 YUAN Qingxin;LIU Kuo;BAO Xuhua;GAO Dongyang;LI He;SONG Jun;ZHOU Zhixin(Key Laboratory of Bovine Disease Control in Northeast China of Ministry of Agriculture and Rural Affairs,College of Animal Science and Veterinary Medicine,Heilongjiang Bayi Agricultural University,Daqing 163319,China;Department of Animal Science and Technology,Heilongjiang Agricultural Economy Vocational College,Mudanjiang 157041,China)

机构地区：[1]黑龙江八一农垦大学动物科技学院农业农村部东北寒区牛病防治重点实验室,大庆163319 [2]黑龙江农业经济职业学院动物科技系,牡丹江157041

出　　处：《畜牧兽医学报》2024年第10期4670-4678,共9页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金青年科学基金项目(31802226);黑龙江省自然科学基金联合引导项目(LH2022C072);黑龙江省牛病防制重点实验室开放课题(PCBD201710)。

摘　　要：本研究旨在阐明白屈菜红碱(chelerythrine,CHE)抗耐甲氧西林金黄色葡萄球菌(methicillin-resistant Staphylococcus aureus,MRSA)活性及探究其抗菌机制。通过测定最小抑菌浓度及最小杀菌浓度分析CHE对MRSA的抗菌活性;检测CHE对MRSA细胞壁及细胞膜的完整性、通透性,结合电镜观察MRSA细胞形态和超微结构,从而对CHE抗菌机制进行初步分析;检测CHE对MRSA菌株活性氧(reactive oxygen species,ROS)水平与ROS相关因子造成的影响,并结合荧光定量及转录组学技术进一步验证CHE抗菌机制。结果表明,CHE对MRSA的最小抑菌浓度为6.25μg·mL^(-1),最小杀菌浓度为12.5μg·mL^(-1)。CHE可通过破坏MRSA菌株细胞细胞壁及细胞膜,发挥抗菌作用。且CHE处理后可抑制MRSA呼吸链SdhABC等基因表达,从而阻断呼吸链电子传递,使ROS水平升高从而发挥抗菌作用。综上,本研究对CHE抗MRSA的抗菌机制进行探究,为临床防治MRSA感染提供新参考。The objective of this study was to investigate the antibacterial effect of chelerythrine(CHE)against methicillin-resistant Staphylococcus aureus(MRSA)and approach its mechanism.The antibacterial activity of CHE against MRSA was determined by minimum inhibitory concentration(MIC)and minimum bactericidal concentration(MBC).After treatment with CHE,the cell membrane and permeability of MRSA were assessed,and the morphology and ultrastructure of MRSA cells were observed by electron microscopy to preliminarily analyze the antibacterial mechanism of CHE.Additionally,the effect of CHE on reactive oxygen species(ROS)levels and ROS-related factors in MRSA strains was detected,combined with fluorescence quantification and transcriptomics to further explore the antibacterial mechanism of CHE.The results showed that the MIC of CHE against MRSA was 6.25μg·mL^(-1),MBC is 12.5μg·mL^(-1),and CHE can destroy cell membrane integrity and permeability to achieve antibacterial effect.CHE may block the electronic transmission of respiratory chain and promote ROS production by inhibiting the expression of gene SdhABC and other genes to exert activity against MRSA.In conclusion,this study explored the antibacterial mechanism of CHE against MRSA,which pro-vided a new reference for clinical prevention of MRSA infection.

关 键 词：耐甲氧西林金黄色葡萄球菌 白屈菜红碱 抗菌机制 氧化应激 

分 类 号：S853.74[农业科学—临床兽医学]