Antimicrobial Resistance of Aeromonas spp.and Vibrio spp.Isolated from Fresh Pangasius Fish in Cambodia  

作　　者：Duk Seyha Chrun Rithy Nguon Samnang Sudsai Trevanich Nora Navarro-Gonzalez Ségolène Calvez Chea Rortana Venn Vutey 

机构地区：[1]Faculty of Agro-Industry,Royal University of Agriculture,Phnom Penh 12400,Cambodia [2]Graduate School,Royal University of Agriculture,Phnom Penh 12400,Cambodia [3]Department of Food Science and Technology,Faculty of Agro-Industry,Kasetsart University,Chatuchak,Bangkok 10900,Thailand [4]The Nantes-Atlantic National College of Veterinary Medicine,Food Science and Engineering(Oniris),INRAE,BIOEPAR,F NATES 65,NATES,France [5]General Directorate of Animal Health and Production,Phnom Penh 120603 Cambodia

出　　处：《Journal of Environmental Science and Engineering(B)》2024年第5期199-209,共11页环境科学与工程（B）

基　　金：supported by grants from HEIP(Higher Education Improvement Project)Project and Royal University of Agriculture.

摘　　要：The study was conducted to identify Aeromonas spp.and Vibrio spp.from fresh Pangasius fish(n=153)in Cambodia and test their antimicrobial susceptibility to antibiotics.The samples were collected from different wet markets of Phnom Penh city and Kampong Thom,and Siem Reap provinces.The bacteria were isolated by using selective medium and their AMR(Antimicrobial Resistance)profile was tested by API 20E technique,respectively.Susceptibility profile was determined for seven antibiotics commonly used.The Vibrio spp.(34.64%,n=53)was found to be higher than Aeromonas spp.(24.83%,n=38).Four Vibrio and four Aeromonas species were identified where V.parahaemolyticus(57%,n=30)was the highest,followed by V.cholerae(38%,n=20),V.fluvialis(3.8%,n=2)and V.aglinolyticus(1.9%,n=1),whereas A.hydrophila(47%,n=18)was the highest,followed by A.hydrophila/caviae(45%,n=17),A.sobria(5%,n=2),A.caviae(2.6%,n=1).All the species presented high multi-resistance to the tested antibiotics.The antibiotic susceptibility profile to ampicillin(74%-100%),ciprofloxacin(7%-100%),sulfamethoxazole/trimethoprim(14%-100%),florfenicol(14%-100%),oxytetracycline(7%-100%),erythromycin(10%-100%)and colistin sulphate(33%-100%)was revealed resistance level in Aeromonas spp.whereas few species of Vibrio spp.resistant to ampicillin(43%-100%),ciprofloxacin(14%-100%),sulfamethoxazole/trimethoprim(14%-100%),florfenicol(14%-100%),oxytetracycline(20%-100%),erythromycin(29%-100%),colistin sulphate(33%-100%)were also identified.The results revealed these Aeromonas spp.and Vibrio spp.are potentially reservoirs of antibiotic resistance genes.MDR(Multidrug Resistance)was widespread among the samples isolated.That is a high-risk source of contamination since those pathogens and antimicrobials are often used.Our findings highlight that the aquatic environment and fresh Pangasius fish act as reservoirs of AMR Aeromonas spp.and Vibrio spp.which underline the need for a judicious use of antimicrobials and timely surveillance of AMR in aquaculture.Overall,the findings of our stud

关 键 词：Aeromonas spp. Vibrio spp. prevalence MDR AMR and Cambodia’s Pangasius. 

分 类 号：Q93[生物学—微生物学]