Phytoene synthases 1 modulates tomato fruit quality through influencing the metabolic flux between carotenoid and flavonoid pathways  

作　　者：Xue Cao Ran Du Yuanchao Xu Yaoyao Wu Keyi Ye Jing Ma Yaqing Lyu Tianshu Sun Xijian Zhu Zhihong Liu Jian Yin Guangtao Zhu Zejun Huang Hongjun Lyu Sanwen Huang Jinzhe Zhang 

机构地区：[1]College of Horticulture,Northwest A&F University,Yangling,Shaanxi 712100,China [2]State Key Laboratory of Vegetable Biobreeding,Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China [3]Shenzhen Branch,Guangdong Laboratory of Lingnan Modern Agriculture,Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs,Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences,Shenzhen,Guangdong 518120,China [4]Institute of Vegetables,Shandong Academy of Agricultural Sciences,Shandong Province Key Laboratory for Biology of Greenhouse Vegetables,Shandong Branch of National Improvement Center for Vegetables,Huang-Huai-Hai Region Scientific Observation and Experimental Station of Vegetables,Ministry of Agriculture and Rural Affairs,Jinan,Shandong 250100,China [5]The AGISCAAS-YNNU Joint Academy of Potato Sciences,Yunnan Normal University,Kunming,Yunnan 650500,China [6]College of Horticulture,Qingdao Agricultural University,Qingdao,Shandong 266109,China

出　　处：《Horticultural Plant Journal》2024年第6期1383-1397,共15页园艺学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(Grant Nos.31991185,31902019,32102384);National Key Research and Development Program of China(Grant No.2021YFF1000103);Key Research and Development Program of Guangdong Province(Grant No.2021B0707010005);Taishan Scholars Program of Shandong Province,China(2016-2020);supported by the Youth innovation Program of Chinese Academy of Agricultural Sciences(Grant No.Y2023QC05)。

摘　　要：The deterioration in fruit quality of commercial tomatoes is a major concern of modern tomato breeding.However,the metabolism and genetics of fruit quality are poorly understood.Here,we performed transgenic and molecular biology experiments to reveal that tomato phytoene synthase 1(SlPSY1)is responsible for the accumulation of an important flavor chemical,6-methyl-5-hepten-2-one(MHO).To dissect the function of SlPSY1 in regulating fruit quality,we generated and analyzed a dataset encompassing over 2000 compounds detected by GC-MS and LC-MS/MS along with transcriptomic data.The combined results illustrated that SlPSY1 deficiency imparts novel flavor to yellow tomatoes with 236 volatiles significantly changed and improves fruit firmness,possibly due to accumulation of seven cutins.Further analysis indicated SlPSY1 is essential for carotenoid-derived metabolite biosynthesis by catalyzing prephytoene-PP(PPPP)to 15-cis-phytoene.Notably,we showed that SlPSY1 can influence the metabolic flux between carotenoid and flavonoid pathways,and this metabolic flux was confirmed by silencing SlCHS1.Our study provided insights into the multiple effects of SlPSY1 on tomato fruit metabolome and highlights the potential to produce high-quality fruit by rational design of SlPSY1 expression.

关 键 词：TOMATO FLAVOR METABOLOME Phytoene synthase 1 CAROTENOID Flavonoid 

分 类 号：S641.2[农业科学—蔬菜学]