乳酸代谢相关的SERPINE1基因与胃癌患者临床预后的关系  

Relationship between SERPINE1 gene related to lactic acid metabolism and clinical prognosis of gastric cancer patients

作　　者：孙锴 王晓东[1] 周轶冰 SUN Kai;WANG Xiaodong;ZHOU Yibing(Second Department of General Surgery,Central Hospital of Jiamusi,Jiamusi,Heilongjiang 154002,China)

机构地区：[1]佳木斯市中心医院普外二科,黑龙江佳木斯154002

出　　处：《国际检验医学杂志》2024年第21期2610-2614,2619,共6页International Journal of Laboratory Medicine

基　　金：黑龙江省卫生健康委员会科研项目(H20200307B)。

摘　　要：目的探讨乳酸代谢相关的SERPINE1基因与胃癌(GC)临床预后的关系。方法该研究纳入了2018年1月至2020年12月在该院接受手术切除治疗的89例GC患者。在初步实验中,该文分析了来自Ⅰ期(n=12)和Ⅳ期(n=12)GC患者的24份血清样本中5个乳酸代谢相关的基因(F5、MTTP、SERPINE1、CYP19A1、SLC52A3)的水平。在第二步中,将初步试验中Ⅳ期GC患者和Ⅰ期GC患者血清中发生显著改变的候选基因在一个更大的独立队列中进行验证,该队列包括65例GC患者和22例健康对照者的血清样本。结果在初步实验中分析的乳酸代谢相关的基因(F5、MTTP、SERPINE1、CYP19A1、SLC52A3)中,与Ⅰ期GC相比,在Ⅳ期患者的血清中SERPINE1显著上调(P<0.001)。GC患者血清SERPINE1的表达水平显著低于健康对照组,差异有统计学意义(P<0.0001)。此外,SERPINE1表达水平随着肿瘤TNM分期的进展而升高(P<0.05)。基于受试者工作特征曲线分析血清SERPINE1对总生存(OS)率的最大预测值[曲线下面积为0.725,截断值为0.0021],将GC患者分为高表达组(n=39)或低表达组(n=26)。与血清SERPINE1低表达组相比,血清SERPINE1高表达组静脉浸润、T3/T4期、淋巴结转移、远处转移例数显著增加,差异有统计学意义(P<0.05)。SERPINE1低表达的GC患者的OS率明显高于SERPINE1高表达的患者,差异有统计学意义(P<0.05)。多变量分析显示,血清SERPINE1高表达(HR=4.51,95%CI:1.23~23.69,P=0.021)是预测GC患者不良预后的独立预后标志物。结论血清乳酸代谢相关的SERPINE1基因可作为GC预后的可靠生物标志物。Objective To explore the relationship between SERPINE1 gene related to lactic acid metabolism and clinical prognosis of gastric cancer(GC).Methods A total of 89 patients with GC who underwent surgical resection in the hospital from January 2018 to December 2020 were enrolled in the study.In the preliminary experiment,the levels of 5 genes related to lactic acid metabolism(F5,MTTP,SERPINE1,CYP19A1,SLC52A3)in 24 serum samples from GC patients with stageⅠ(n=12)and stageⅣ(n=12)were analyzed.In the second step,the candidate genes with significant changes in serum of GC patients with stageⅣand GC patients with stageⅠin the preliminary experiment were verified in a larger independent cohort,which included serum samples of 65 GC patients and 22 healthy controls.Results Among the genes related to lactic acid metabolism(F5,MTTP,SERPINE1,CYP19A1,SLC52A3)analyzed in the preliminary experiment,SERPINE1 was significantly up-regulated in the serum of patients with stageⅣcompared with that in the serum of patients with stageⅠ(P<0.001).The expression level of SERPINE1 in serum of GC patients was significantly lower than that of healthy controls(P<0.0001).In addition,the expression level of SERPINE1 increased with the progress of TNM staging(P<0.05).Based on the receiver operating characteristic curve,the maximum predictive value of SERPINE1 on overall survival(OS)rate was analyzed(area under the curve=0.725,cut off value=0.0021),and GC patients were divided into high expression group(n=39)or low expression group(n=26).Compared with the SERPINE1 low expression group,the cases of venous infiltration,T3/T4 stage,lymph node metastasis and distant metastasis in the SERPINE1 high expres-sion group increased,and the differences were statistically significant(P<0.05).The OS rate of GC patients with low expression of SERPINE1 was significantly higher than that of patients with high expression of SER-PINE1(P<0.05).Multivariate analysis showed that the high expression of SERPINE1 in serum(HR=4.51,95%CI=1.23-23.69,P=0.021)was an

关 键 词：胃癌 乳酸 临床预后 血清 

分 类 号：R735.2[医药卫生—肿瘤]