果胶酶苎麻脱胶:一种生产还原糖的新途径  

Degumming Ramie by Pectinase:a New Way to Produce Reducing Sugar

作　　者：马世航 成莉凤[1] 冯湘沅[1] 杨琦[1] 席果果 彭正红 段盛文[1] MA Shihang;CHENG Lifeng;FENG Xiangyuan;YANG Qi;XI Guoguo;PENG Zhenghong;DUAN Shengwen(Institute of Bast Fiber Crops,Chinese Academy of Agricultural Sciences,Changsha 410221,Hunan,China)

机构地区：[1]中国农业科学院麻类研究所,湖南长沙410221

出　　处：《中国麻业科学》2024年第5期291-297,共7页Plant Fiber Sciences in China

基　　金：中国农业科学院科技创新工程项目(ASTIP-IBFC-05);财政部和农业农村部:国家麻类产业技术体系资助(CARS-16-E22);国家自然科学基金(32301281);湖南省自然科学基金项目(2023JJ50315;2023JJ30621);所级“岳麓青年人才”项目(IBFC-YLQN-202102);长沙市自然科学基金项目(kq2208250;kq2208247);新疆维吾尔自治区区域协同创新专项(科技援疆计划)项目(2022E2105)。

摘　　要：为提高苎麻生物质利用效率,使用果胶酶对苎麻韧皮进行水解,通过Box-Behnken响应面法优化果胶酶水解苎麻果胶工艺,采用3,5-二硝基水杨酸(DNS)法检测果胶水解液中的还原糖含量。结果表明:还原糖最佳提取工艺为水解温度52℃,果胶酶浓度4.8%,水解时间7 h。在该条件下,水解液中还原糖含量可达(10.32±0.47)mg/mL。该工艺适用于将苎麻韧皮中果胶组分转化为可溶性还原糖,为后续生物质资源再利用提供依据。To improve the utilisation efficiency of ramie biomass,this study uses pectinase to hydrolyse ramie bast.The Box-Behnken response surface methodology was used to optimise the pectinase hydrolysis process of ramie pectin.The reducing sugar content in the pectin hydrolysate was determined by using the 3,5-dinitrosalicylic acid(DNS)method.The optimal process for extracting the reducing sugar content was found to be a hydrolysis temperature of 52℃,a pectinase concentration of 4.8%,and a hydrolysis time of 7 h.Under these conditions,the reducing sugar content of the hydrolysate could reach(10.32±0.47)mg/mL.This process is technically reliable and suitable for converting ramie pectin into reducing sugar and provides a basis for the subsequent reuse of biomass resources.

关 键 词：苎麻 果胶 响应面法 还原糖含量 

分 类 号：S563.1[农业科学—作物学]