Characterization of N6-methyladenosine long non-coding RNAs in sporadic congenital cataract and age-related cataract  

作　　者：Hong-Fei Ye Xiang Zhang Zhen-Nan Zhao Ce Zheng Ping Fei Yu Xu Jiao Lyu Ji-Li Chen Xun-Xiang Guo Huang Zhu Pei-Quan Zhao 

机构地区：[1]Department of Ophthalmology,Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine,Shanghai 200092,China [2]Department of Ophthalmology and Vision Science,Eye Ear Nose and Throat Hospital of Fudan University,Shanghai 200031,China [3]Department of Ophthalmology,Shanghai Shibei Hospital of Jing’an District,Shanghai 200040,China [4]Key Laboratory of Systems Biomedicine(Ministry of Education),Shanghai Center for Systems Biomedicine,Shanghai Jiao Tong University,Shanghai 200030,China

出　　处：《International Journal of Ophthalmology(English edition)》2024年第11期1973-1986,共14页国际眼科杂志（英文版）

基　　金：Supported by the National Natural Science Foundation of China(No.82171069;No.82371070);Shanghai Science and Technology Committee(No.22015820200);Shanghai Municipal Health Commission Innovative Medical Device Application Demonstration Project(No.23SHS03500-03);Project of Shanghai Municipal Commission of Health and Family Planning(No.202140224);Grants from Interdisciplinary Program of Shanghai Jiao Tong University(No.YG2021QN52).

摘　　要：AIM:To characterize the N6-methyladenosine(m6A)modification patterns in long non-coding RNAs(lncRNAs)in sporadic congenital cataract(CC)and age-related cataract(ARC).METHODS:Anterior capsule of the lens were collected from patients with CC and ARC.Methylated RNA immunoprecipitation with next-generation sequencing and RNA sequencing were performed to identify m6A-tagged lncRNAs and lncRNAs expression.Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses and Gene Ontology annotation were used to predict potential functions of the m6A-lncRNAs.RESULTS:Large amount of m6A peaks within lncRNA were identified for both CC and ARC,while the level was much higher in ARC(49870 peaks)than that in CC(18688 peaks),yet those difference between ARC in younger age group(ARC-1)and ARC in elder age group(ARC-2)was quite slight.A total of 1305 hypermethylated and 1178 hypomethylated lncRNAs,as well as 182 differential expressed lncRNAs were exhibited in ARC compared with CC.On the other hand,5893 hypermethylated and 5213 hypomethylated lncRNAs,as well as 155 significantly altered lncRNA were identified in ARC-2 compared with ARC-1.Altered lncRNAs in ARC were mainly associated with the organization and biogenesis of intracellular organelles,as well as nucleotide excision repair.CONCLUSION:Our results for the first time present an overview of the m6A methylomes of lncRNA in CC and ARC,providing a solid basis and uncovering a new insight to reveal the potential pathogenic mechanism of CC and ARC.

关 键 词：congenital cataract age-related cataract N6-methyladenosine RNA modification long non-coding RNA EPIGENETICS 

分 类 号：R776.1[医药卫生—眼科]