响应面试验优化功能性红曲米发酵工艺  

Optimization of Fermentation Technology of Functional Red Kojic Rice by Response Surface Methodology

作　　者：吴曼 陈婷婷 黄秋月 徐筱雨 谢新月 陈庆 吴鹏飞 WU Man;CHEN Tingting;HUANG Qiuyue;XU Xiaoyu;XIE Xinyue;CHEN Qing;WU Pengfei(College of Life Science and Technology,Yantze Normal University,Chongqing 408100,China)

机构地区：[1]长江师范学院生命科学与技术学院,重庆408100

出　　处：《农产品加工》2024年第20期48-52,共5页Farm Products Processing

基　　金：重庆市教育委员会科学技术研究计划项目(KJQN202001424,KJQN202201443)。

摘　　要：以实验室筛选并保藏的红曲菌为试验菌种,首先通过Plackett-burman试验筛选出对红曲菌产洛伐他汀具有显著性影响的因素,再应用最陡爬坡法确定最大响应区域,最后利用Box-behnken试验设计和响应面分析法得到红曲菌高产洛伐他汀的最佳工艺条件。结果表明,通过P-B试验筛选出接种量、柠檬酸添加量、谷氨酸添加量为影响洛伐他汀含量的显著因素,响应面优化得出的最佳工艺条件为接种量19%,柠檬酸添加量1.4%,谷氨酸添加量0.9%,此时功能性红曲米洛伐他汀含量为12.23 mg/g。In this study,one red kojic rice strain which was selected and preserved by our laboratory was used as experimental strain.Firstly,the significantly factors of lovastatin production by red kojic rice was screened by Plackett-burman experiment,and then the maximum response area was determined by steepest ascent experiment.Finally,the optimal process conditions for high-yield lovastatin production by red kojic rice was obtained by Box-behnken experimental design and response surface analysis.The results showed that the inoculation amount,citric acid addition and glutamic acid addition were selected as significant factors affecting the content of lovastatin through P-B experiment.The optimal process conditions optimized by response surface methodology were 19%inoculation amount,1.4%citric acid addition,and 0.9%glutamic acid addition.At these conditions,the lovastatin content of functional red kojic rice reached to 12.23 mg/g.

关 键 词：功能性红曲米 洛伐他汀 固态发酵 响应面分析 

分 类 号：TQ925.7[轻工技术与工程—发酵工程]