海南地区大米中硒含量及形态分析  

Analysis of the selenium content and speciation in rice in Hainan Province

作　　者：谷满屯 林志藩 韦盈 康蕊 王丽强 符郁馥 周玉玲 GU Man-Tun;LIN Zhi-Fan;WEI Ying;KANG Rui;WANG Li-Qiang;FU Yu-Fu;ZHOU Yu-Ling(Institute of Food Testing,Hainan Academy of Inspection and Testing,Key Laboratory of Tropical Fruits and Vegetables Quality and Safety,State Administration for Market Regulation,Haikou 570100,China)

机构地区：[1]海南省检验检测研究院食品检验检测中心,国家市场监督管理总局重点实验室(热带果蔬质量与安全),海口570100

出　　处：《食品安全质量检测学报》2024年第18期76-83,共8页Journal of Food Safety and Quality

基　　金：海南省自然科学基金青年基金项目(320QN375);国家市场监督管理总局重点实验室(热带果蔬质量与安全)自主研究课题项目(ZZ-2024012)。

摘　　要：目的研究海南地区大米中硒含量与存在形态情况。方法利用电感耦合等离子体串联质谱仪在氧气反应模式(O_(2)模式)下,测定海南地区大米的总硒含量;利用高效液相色谱-电感耦合等离子体串联质谱仪的O_(2)模式,测定海南地区大米中硒形态含量。结果相较于常规的电感耦合等离子体质谱仪,电感耦合等离子体串联质谱仪在O_(2)模式下,以最高丰度同位素^(80)Se为检测目标质量数,其检测大米中硒的含量更加准确。228批次海南地区大米硒含量平均值为(0.0484±0.0728)mg/kg,约42%的大米达到GB/T 22499—2008《富硒稻谷》规定的富硒大米标准。随机对50批海南富硒大米的硒形态进行分析,检出的硒形态主要为硒代蛋氨酸,少量大米检出硒代胱氨酸,其中硒代蛋氨酸(以Se计)的含量范围为0.0024~0.0255 mg/kg,占总硒含量的百分比为1.7%~51.4%。结论海南地区大米具有一定的硒含量基础,部分大米硒代蛋氨酸含量占比较高,可开发成优质的富硒大米。Objective To study the content and speciation of selennium in rice in Hainan Province.Methods The total selenium content of rice in Hainan Province was determined by inductively coupled plasma tandem mass spectrometry in oxygen reaction mode(O_(2) mode).The selenium speciation in rice in Hainan Province was determined by high performance liquid chromatography-inductively coupled plasma tandem mass spectrometry in O_(2) mode.Results Compared with conventional inductively coupled plasma mass spectrometry,inductively coupled plasma tandem mass spectrometry in O_(2) mode,with the highest abundance isotope ^(80)Se as the detection target mass number,was more accurate in detecting the selenium content in rice.The average selenium content in 228 batches of rice in Hainan was(0.0484±0.0728)mg/kg,and about 42% of the rice met the Se-enriched rice standard specified in the GB/T 22499—2008 Selenium-enriched rice.The selenium speciation of 50 batches of Hainan Se-enriched rice were randomly analyzed.The selenium speciation detected were mainly selenomethionine,and selenocystine was detected in a small amount of rice.The content of selenomethionine(calculated as Se)ranged from 0.0024 to 0.0255 mg/kg,accounting for 1.7% to 51.4%of the total selenium content.Conclusion The rice in Hainan Province has a certain selenium content basis,and some of the rice has a high content of selenomethionine,which can be developed into high-quality Se-enriched rice.

关 键 词：大米 电感耦合等离子体串联质谱法 总硒 硒形态 硒代蛋氨酸 

分 类 号：O657.63[理学—分析化学] TS213.3[理学—化学]