枯草芽孢杆菌固体发酵板蓝根药渣制备多糖饲料添加剂的工艺优化  

Optimization of preparation of polysaccharide feed additive by solid fermentation of Radix isatidis residue by Bacillus subtilis

作　　者：杨英歌[1] 王娟[1] 王丹[1] YANG Ying-ge;WANG Juan;WANG Dan

机构地区：[1]徐州生物工程职业技术学院,江苏徐州221006

出　　处：《饲料研究》2024年第18期88-94,共7页Feed Research

基　　金：徐州市科技项目(项目编号:KC22014)。

摘　　要：为了高值化利用板蓝根药渣,试验优化了固体发酵板蓝根药渣制备多糖饲料添加剂的工艺,并对其抗氧化能力进行了研究。结果显示,从28株枯草芽孢杆菌中筛选得到高产纤维素酶菌株Bacillus subtilis YBS-18。通过单因素试验和响应面优化Bacillus subtilis YBS-18固体发酵板蓝根药渣生产多糖的工艺,确定最佳工艺为:液料比1.2 mL/g、装料量0.3 g/mL、葡萄糖浓度8%、硫酸铵浓度4.5%、接种量4.5%、发酵温度38℃。在最佳发酵工艺条件下,多糖含量达到28.17 mg/g。产物多糖对1,1-二苯基-2-三硝基苯肼(DPPH)自由基、羟基自由基、超氧阴离子自由基表现出较好的清除能力,清除率随着多糖浓度的提高而上升。研究表明,此工艺提升了板蓝根药渣的营养价值,为板蓝根药渣饲料添加剂的开发和应用提供理论基础。In order to utilize Radix isatidis residue in high value,the solid fermentation process from Radix isatidis residue to produce polysaccharide feed additive was optimized,and its antioxidant capacity was studied.The results showed that the high-yielding cellulase strain Bacillus subtilis YBS-18 was isolated from 28 strains.The production process of polysaccharide from solid fermentation of Bacillus subtilis YBS-18 isatis root residue was optimized by single factor test and response surface.The optimal process was determined as follows:The liquid-solid ratio was 1.2 mL/g,the loading rate was 0.3 g/mL,the glucose concentration was 8%,the ammonium sulfate concentration was 4.5%,the inoculation rate was 4.5%,and the fermentation temperature was 38℃.Under the optimum fermentation conditions,the polysaccharide content reached 28.17 mg/g.The polysaccharide showed good scavenging ability for DPPH free radicals,hydroxyl free radicals and superoxide anion free radicals,and the scavenging rate increased with the increase of polysaccharide concentration.The results show that the process improved the nutritional value of Radix isatidis residue and provided a theoretical basis for the development and application of feed additives of Radix isatidis residue.

关 键 词：枯草芽孢杆菌 板蓝根药渣 固体发酵 多糖 抗氧化 

分 类 号：S816.34[农业科学—饲料科学]