化橘红提取物的制备工艺优化及其降脂作用  

Optimization of the Preparation of Exocarpium Citri grandis Extract and Its Lipid Level-lowering Effects

作　　者：李锦坤 金刚亮 王艳慧 冼少华 赵俊鑫 余俊朗 刘浩帆 谭耀康 温骏炜 熊平 LI Jinkun;JIN Gangliang;WANG Yanhui;XIAN Shaohua;ZHAO Junxin;YU Junlang;LIU Haofan;TAN Yaokang;WEN Junwei;XIONG Ping(College of Materials and Energy,South China Agricultural University,Guangzhou 510642,China;Guangzhou Xiangxue Pharmaceutical Co.Ltd.,Guangzhou 510663,China;Sinopharm Group Dezhong(Foshan)Pharmaceutical Co.Ltd.,Foshan 528000,China)

机构地区：[1]华南农业大学材料与能源学院,广东广州510642 [2]广州市香雪制药股份有限公司,广东广州510663 [3]国药集团德众(佛山)药业有限公司,广东佛山528000

出　　处：《现代食品科技》2024年第10期250-258,共9页Modern Food Science and Technology

基　　金：国家重点研发计划项目(2018YFC1706702)。

摘　　要：采用单因素试验和L_(9)(3^(4))正交试验设计法,以加水量、提取次数、提取时间和提取温度为考察对象,以化橘红柚皮苷质量分数和干浸膏质量分数为考察指标,确定化橘红最佳提取工艺参数;并通过饲喂高脂饲料建立混合型高脂血症小鼠模型,探讨化橘红提取物对实验小鼠高脂血症的降脂作用。结果表明,化橘红水提物的优选制备工艺参数为:以水作为溶媒,料液比1:30,提取温度70℃,提取3次,每次60min。在此工艺条件下,化橘红水提物柚皮苷质量分数为56.15 mg/g,传统工艺主要以乙醇为溶媒,此工艺可为化橘红水提提供参考。与模型组相比,化橘红高剂量组胆固醇(TC)、甘油三脂(TG)和低密度脂蛋白胆固醇(LDL-C)水平分别降低了47.11%,36.84%和65.18%,肝指数和Lee’s指数显著降低,血清谷丙转氨酶(ALT)和谷草转氨酶(AST)水平分别降低了56.68%和56.63%,且可以改善肝细胞肿胀变性,减轻脂滴沉积以及脂肪空泡形成。结果说明,化橘红提取物对混合型高脂血症模型小鼠有降脂作用。The optimal extraction conditions of exocarpium Citri grandis were determined using a single-factor test and an orthogonal test.The effects of water addition,extraction frequency,extraction time,and extraction temperature were investigated;the mass fractions of naringin and dry extract were used as evaluation indexes.Furthermore,a combined hyperlipidemia mouse model was established by feeding mice a high-fat diet to investigate the lipid level-lowering effects of exocarpium Citri grandis extract.The results showed that the optimal conditions for preparing the aqueous extract of exocarpium Citri grandis were as follows:solvent,water;solid-liquid ratio,1:30;and extraction temperature,70℃;the extraction was performed three times(60 minutes each time).Under these conditions,the naringin content in the aqueous extract of exocarpium Citri grandis was 56.15 mg/g.Traditional processes mainly use ethanol as the solvent;this process can provide a reference for aqueous extraction of Exocarpium Citri Grandis.The high-dose exocarpium Citri grandis group showed 47.11%,36.84%,and 65.18%lower levels of TC,TG,and LDL-C,respectively,significantly lower liver index and Lee's index values,and 56.68%and 56.63%lower serum ALT and AST levels,respectively,than the model group.The high-dose group also showed milder liver cell swelling and degeneration and reduced lipid droplet deposition and fat vacuole formation.The results indicate that the exocarpium Citri grandis extract exerts lipid level-lowering effects on mice with combined hyperlipidemia.

关 键 词：化橘红 提取物 工艺优化 降血脂 

分 类 号：TS201.4[轻工技术与工程—食品科学]