广西产香茅草精油在不同蒸馏时段的化学成分及抗氧化活性  

Chemical Composition and Antioxidant Activity of Essential Oil Collected at Different Distillation Periods from Cymbopogon citratus(DC)Stapf in Guangxi

作　　者：何玲利 张倩[1] 凌玉钊 陈房姣 侯方域 李梦丹 田玉红[1] HE Lingli;ZHANG Qian;LING Yuzhao;CHEN Fangjiao;HOU Fangyu;LI Mengdan;TIAN Yuhong(College of Biological and Chemical Engineering,Guangxi University of Science and Technology,Liuzhou 545006,Guangxi,China;Guangxi G.Taste Food Co.,Ltd.,Liuzhou 545001,Guangxi,China;College of Medicine,Guangxi University of Science and Technology,Liuzhou 545006,Guangxi,China)

机构地区：[1]广西科技大学生物与化学工程学院,广西柳州545006 [2]广西桂味联食品有限公司,广西柳州545001 [3]广西科技大学医学部,广西柳州545006

出　　处：《食品研究与开发》2024年第21期10-17,共8页Food Research and Development

基　　金：国家自然科学基金项目(22268012);广西自然科学基金项目(2021GXNSFAA220067)。

摘　　要：以不同蒸馏时段的香茅草精油为研究对象,通过水蒸气蒸馏法提取,气相色谱-质谱(gas chromatographymass spectrometry,GC-MS)法进行成分分析,采用DPPH自由基清除法、ABTS^(+)自由基清除法对抗氧化能力进行评价。结果表明,在0~10、10~30、30~60、60~360 min蒸馏时段精油分别鉴定出32、35、34、34种成分。各蒸馏阶段的主要成分及相对含量不同,0~10、10~30、30~60 min是橙花醛(35.33%、31.58%、28.47%)、香叶醛(38.50%、32.99%、37.56%),60~360 min蒸馏时段是正十六酸(18.24%)、橙花醛(8.49%)、香叶醛(12.05%)。不同蒸馏时段精油清除DPPH自由基的IC_(50)值分别为16.05、7.89、4.03、1.47 mg/mL,清除ABTS^(+)自由基的IC_(50)值分别为3.58、2.40、1.08、0.55 mg/mL。综上所述,香茅草精油具有较强的抗氧化活性,在60~360 min蒸馏时段精油对DPPH自由基和ABTS^(+)自由基的清除能力最强。This paper studied the essential oil collected at different distillation periods from Cymbopogon citratus(DC)Stapf.The essential oil was extracted by steam distillation and analyzed by gas chromatography-mass spectrometry(GC-MS).The antioxidant ability of essential oil were evaluated by DPPH radical scavenging method and ABTS^(+)radical cation scavenging method.It was found that 32,35,34 and 34 components of essential oil were identified in 0-10,10-30,30-60 min and 60-360 min distillation stages respectively.The main components and their relative content were different in each distillation stage.0-10,10-30,30-60 min distillation periods were rich in neral(35.33%,31.58%,28.47%)and geranial(38.50%,32.99%,37.56%).The major components in 60~360 min distillation periods were n-hexadecanoic acid(18.24%),neral(8.49%)and geranial(12.05%).The IC_(50) values of essential oil for scavenging DPPH radical were 16.05,7.89,4.03 mg/mL and 1.47 mg/mL,respectively,during different distillation periods.The IC_(50) values for ABTS^(+)radical scavenging were 3.58,2.40,1.08 mg/mL and 0.55 mg/mL,respectively.In conclusion,the essential oil of Cymbopogon citratus(DC)Stapf possessed strong antioxidant activities,and the essential oil collected at 60~360 min showed the strongest scavenging activities on DPPH and ABTS^(+)free radicals.

关 键 词：香茅草 精油 蒸馏时段 化学成分 抗氧化活性 

分 类 号：TQ654.2[化学工程—精细化工] O657.63[理学—分析化学]