黄精多糖中果糖含量的精确测定方法  

Precise Determination of Fructose Content in Polygonatum sibiricum Polysaccharides

作　　者：刘俊佚 郑义 刘伟 袁涛 闫竞宇 LIU Junyi;ZHENG Yi;LIU Wei;YUAN Tao;YAN Jingyu(Xinjiang Key Laboratory of Clean Conversion and High Value Utilization of Biomass Resource,Yili Normal University,Yining 835000,Xinjiang,China;Ganjiang Chinese Medicine Innovation Center,Nanchang 330100,Jiangxi,China;Dalian Institute of Chemical Physics,Chinese Academy of Sciences,Dalian 116023,Liaoning,China)

机构地区：[1]伊犁师范大学新疆生物质资源清洁转化与高值化利用重点实验室,新疆伊宁835000 [2]赣江中药创新中心,江西南昌330100 [3]中国科学院大连化学物理研究所,辽宁大连116023

出　　处：《食品研究与开发》2024年第21期164-171,共8页Food Research and Development

基　　金：江西省“双千计划”项目;江西省自然科学基金资助项目(20232BAB21502)。

摘　　要：为准确测定黄精多糖中果糖的含量,采用亲水色谱-电雾式检测分析(hydrophilic interaction liquid chromatography-charged aerosol detector,HILIC-CAD)法,系统地考察多糖浓度、酸浓度、水解温度及水解时间4个多糖水解单因素,并结合正交试验获得黄精多糖的最佳水解条件。结果表明,黄精多糖的最佳水解条件为1.0 mg/mL黄精多糖在0.100 mol/L三氟乙酸溶液中,于80℃水解30 min,果糖可被完全释放,且未转变为其他物质。采用HILIC-CAD结合柱前衍生-反相色谱法,测定山东、安徽、甘肃、四川和陕西5个产地黄精多糖中果糖及其它6种单糖的含量,其中安徽产地的黄精多糖果糖含量较低(51.74%),其它4个产地黄精多糖中果糖含量较高且含量相似(78.71%~83.08%)。This study aimed to accurately determine the fructose content in Polygonatum sibiricum polysaccharides.A hydrophilic interaction liquid chromatography-charged aerosol detector(HILIC-CAD)method was employed to systematically investigate the four single factors on polysaccharide hydrolysis,i.e.,polysaccharide concentration,acid concentration,temperature,and hydrolysis time.Optimal hydrolysis conditions were determined through orthogonal testing.The results indicated that the optimal conditions for the hydrolysis of P.sibiricum polysaccharides were as follows:1.0 mg/mL P.sibiricum polysaccharides were hydrolyzed in a 0.100 mol/L trifluoroacetic acid solution at 80℃ for 30 min.Under these conditions,fructose was completely released without conversion to other substances.By combining HILIC-CAD with pre-column derivatization and reverse-phase chromatography,the exact content of fructose and six other monosaccharides in P.sibiricum polysaccharides from five different regions(Shandong,Anhui,Gansu,Sichuan,and Shaanxi)was determined.It was found that the fructose content in the P.sibiricum polysaccharides from Anhui was relatively low(51.74%),while the fructose content from the other four regions was higher and similar(78.71%-83.08%).

关 键 词：黄精多糖 果糖含量 亲水色谱-电雾式检测分析 单糖组成分析 酸水解 

分 类 号：TS201.2[轻工技术与工程—食品科学]