Characterization of HpnG as a Purine Nucleoside Phosphorylase in Bacteriohopanepolyol Biosynthesis  

作　　者：Xinhui Li Xiaoyu Zhu Yuting Zhong Wei Zhang Fener Chen Wenning Wang Wei Ding Qi Zhang 

机构地区：[1]Department of Chemistry,Fudan University,Shanghai 200433,China [2]State Key Laboratory of Microbial Metabolism,School of Life Sciences&Biotechnology,Shanghai Jiao Tong University,Shanghai 200240,China [3]Key Laboratory of Extreme Environmental Microbial Resources and Engineering of Gansu Province,Northwest Institute of Eco-Environment and Resources,Chinese Academy of Sciences,Lanzhou,Gansu 73000,China

出　　处：《Chinese Journal of Chemistry》2024年第19期2351-2356,共6页中国化学（英文版）

基　　金：supported by grants from the National Key Research andDevelopmenttProgram(2018YFA0900402,2021YFA0910501);the National Natural Science Foundation of China(21921003,32270070,32270050,U22A20451);the Funding of Innovative Research Team of High-Level Local Universities in Shanghai and a key laboratory program of the Education Commission of Shanghai Municipality(ZDSYS14005);West Light Foundation of The Chinese Academy of Sciences(xbzg-zdsys-202105).

摘　　要：Comprehensive Summary HpnG plays a crucial role in the production of ribosylhopane,a key intermediate in the biosynthesis of bacteriohopanepolyol.Despite early extensive studies,the precise function of HpnG has remained elusive.Here,we report functional characterization of HpnG as a purine nucleoside phosphorylase,which converts adenosylhopane to phosphoribosylhopane in the presence of phosphate.HpnG demonstrates broad substrate specificity and impressive stability,making it a valuable enzymatic tool for applications in nucleoside processing and related biotechnology.

关 键 词：ENZYMES Enzyme catalysis NUCLEOSIDES BIOSYNTHESIS Natural products LIPIDS Phosphorolysis BIOTRANSFORMATIONS 

分 类 号：O62[理学—有机化学]