Morphological Tracing and Functional Identification of Monosynaptic Connections in the Brain:A Comprehensive Guide  

作　　者：Yuanyuan Li Yuanyuan Fang Kaiyuan Li Hongbin Yang Shumin Duan Li Sun 

机构地区：[1]Affiliated Mental Health Center and Hangzhou Seventh People’s Hospital and School of Brain Science and Brain Medicine,Zhejiang University School of Medicine,Hangzhou,310058,China [2]Liangzhu Laboratory,MOE Frontier Science Center for Brain Science and Brain-machine Integration,State Key Laboratory of Brain-machine Intelligence,Zhejiang University,Hangzhou,311121,China [3]NHC and CAMS Key Laboratory of Medical Neurobiology,Zhejiang University,Hangzhou,310058,China [4]Department of Neurobiology and Department of Neurology of the Second Affiliated Hospital,Zhejiang University School of Medicine,Hangzhou,310058,China [5]Institute of Basic Medical Sciences,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing,100005,China

出　　处：《Neuroscience Bulletin》2024年第9期1364-1378,共15页神经科学通报（英文版）

基　　金：National Key Research and Development Program of China(2022YFC2402703);Scientific and Technological Innovation 2030 Program of China,major projects(2021ZD0200408);National Natural Science Foundation of China(32171007 and 31800880);Non-profit Central Research Institute Fund of the Chinese Academy of Medical Sciences(2018PT31041);Science and Technology Planning Project of Guangdong Province(2018B030331001);Fundamental Research Funds for the Central Universities(K20210089).

摘　　要：Behavioral studies play a crucial role in unraveling the mechanisms underlying brain function.Recent advances in optogenetics,neuronal typing and labeling,and circuit tracing have facilitated the dissection of the neural circuitry involved in various important behaviors.The identification of monosynaptic connections,both upstream and downstream of specific neurons,serves as the foundation for understanding complex neural circuits and studying behavioral mechanisms.However,the practical implementation and mechanistic understanding of monosynaptic connection tracing techniques and functional identification remain challenging,particularly for inexperienced researchers.Improper application of these methods and misinterpretation of results can impede experimental progress and lead to erroneous conclusions.In this paper,we present a comprehensive description of the principles,specific operational details,and key steps involved in tracing anterograde and retrograde monosynaptic connections.We outline the process of functionally identifying monosynaptic connections through the integration of optogenetics and electrophysiological techniques,providing practical guidance for researchers.

关 键 词：Trans-monosynaptic RETROGRADE ANTEROGRADE Viral tool Neural circuit OPTOGENETICS ELECTROPHYSIOLOGY 

分 类 号：R74[医药卫生—神经病学与精神病学]