STED Imaging of Vesicular Endocytosis in the Synapse  

作　　者：Shaoqin Hu Zhenli Xie Bianbian Wang Yang Chen Zexin Jing Ying Hao Jingyu Yao Xuanang Wu Jingxiao Huo Anqi Wei Yuhao Qin Nan Dong Chaowen Zheng Qian Song Jiangang Long Xinjiang Kang Changhe Wang Huadong Xu 

机构地区：[1]Neuroscience Research Center,Key Laboratory of Biomedical Information Engineering of Ministry of Education,School of Life Science and Technology,Department of Neurology,the First Affiliated Hospital,Core Facilities Sharing Platform,Xi’an Jiaotong University,Xi’an,710049,China [2]College of Life Sciences,Liaocheng University,Liaocheng,252059,China [3]Key Laboratory of Medical Electrophysiology,Ministry of Education of China,Collaborative Innovation Center for Prevention and Treatment of Cardiovascular Disease,and the Institute of Cardiovascular Research,Southwest Medical University,Luzhou,646000,China

出　　处：《Neuroscience Bulletin》2024年第9期1379-1395,共17页神经科学通报（英文版）

基　　金：National Natural Science Foundation of China(32171233,81901308,81974203,32300819,31670843,32000704,21790390,and 21790394);Key Research and Development Program of Shaanxi Province of China(2023-ZDLSF-23);Natural Science Foundation of Shaanxi Province of China(2019JC-07,2020JQ-029,and 2023-JC-QN-0236);Natural Science Foundation of Sichuan Province of China(2020YJ0337 and 2020YJ0378);Sichuan Science and Technology Program(2022YFS0615);China Postdoctoral Science Foundation(2018M640972);Innovation Capability Support Program of Shaanxi Province,China(2021TD-37);The Shaanxi Postdoc Funding(2023BSHTBZZ15,2023BSHYDZZ39 and 2023BSHEDZZ67).

摘　　要：Endocytosis is a fundamental biological process that couples exocytosis to maintain the homeostasis of the plasma membrane and sustained neurotransmission.Super-resolution microscopy enables optical imaging of exocytosis and endocytosis in live cells and makes an essential contribution to understanding molecular mechanisms of endocytosis in neuronal somata and other types of cells.However,visualization of exo-endocytic events at the single vesicular level in a synapse with optical imaging remains a great challenge to reveal mechanisms governing the synaptic exo-endocytotic coupling.In this protocol,we describe the technical details of stimulated emission depletion(STED)imaging of synaptic endocytosis at the single-vesicle level,from sample preparation and microscopy calibration to data acquisition and analysis.

关 键 词：ENDOCYTOSIS STED imaging Synaptic vesicle Single-vesicle level 

分 类 号：R741[医药卫生—神经病学与精神病学]