基于响应面法的非洲哈茨木霉AD-G4菌株固体发酵条件优化  

Optimization of solid-state fermentation conditions of Trichoderma afroharzianum AD-G4 strain using response surface method

作　　者：包雨微 郝宁科 贠新龙 彭良忠 刘香萍[1] BAO Yu-wei;HAO Ning-ke;YUN Xin-long;PENG Liang-zhong;LIU Xiang-ping(College of Animal Science and Technology,Heilongjiang Bayi Agricultural University,Daqing 163319,China)

机构地区：[1]黑龙江八一农垦大学动物科技学院,黑龙江大庆163319

出　　处：《草原与草坪》2024年第5期33-42,共10页Grassland and Turf

基　　金：科技创新2030—重大项目(2022ZD04012);黑龙江省饲草饲料现代农业产业技术协同创新推广体系。

摘　　要：【目的】为了获得非洲哈茨木霉AD-G4固体发酵的最佳条件。【方法】利用单因素试验、BBD(Box-Behnken Design)响应面试验对非洲哈茨木霉AD-G4菌株的固体发酵培养基、发酵条件等影响因素进行了优化。【结果】适合此菌株产生的最佳培养基组成为:燕麦粉和小麦秸秆1∶1;最佳培养条件为初始p H值7.0,含水量52%,孢子浓度5.40×10^(6)个/m L,温度28℃,培养天数为8 d。【结论】在优化的培养条件下,非洲哈茨木霉AD-G4分生孢子产量达到1.91×10^(10)个/g。通过培养条件优化确定了适合非洲哈茨木霉AD-G4菌株产生分生孢子的条件,为高效开发非洲哈茨木霉生防菌剂奠定了基础。【Objective】To determine the optimal formulafor solidstate fermentation of Trichoderma afroharzianum ADG4.【Method】This study employed single factor test and BoxBehnken Design(BBD)response surface methodologyto optimize the solid fermentation medium and conditions for the T.afroharzianum ADG4 strain.【Result】The findings indicated that the optimal medium composition consisted of a 1∶1 ratio of oat flour to wheat straw.The ideal culture conditions were determined to be an initial pH of 7.0,water content of 52%,spore concentration of 5.40×10^(6)spores/mL,a temperature of 28℃,and a culture durationof 8 days.【Conclusion】Under these culture conditions,conidia yield of T.afroharzianum ADG4 reached 1.91×10^(10)spores/g.The optimized culture conditions provide a foundation for the development of efficient T.afroharzianum biocontrol agent.

关 键 词：非洲哈茨木霉 固体发酵 分生孢子 发酵条件 响应面 优化 

分 类 号：S54[农业科学—作物学] S476