孤独症、智力障碍患儿血液中的氧化应激水平分析  

Analysis of oxidative stress levels in blood of children with autism and intellectual disability

作　　者：白璐 贾含香 李颖[6] 王娟[7] 周泓 谢芳[6] 王军[1,2,3,4,5] BAI Lu;JIA Hanxiang;LI Ying;WANG Juan;ZHOU Hong;XIE Fang;WANG Jun(The Ministry of Industrial Fermentation Jointly Established A Collaborative Innovation Center,Hubei University of Technology,Wuhan 430068;Cell Regulation and Molecular Pharmacy Innovation Introduction Base(“111 Base”),Hubei University of Technology,Wuhan 430068;Institute for Diagnosis and Intervention of Autism and Depression,Hubei University of Technology,Wuhan 430068;International Joint Research Center for Health and Precision Medicine and Nutrition,Hubei University of Technology,Wuhan 430068;Department of Bioengineering and Food,Hubei University of Technology,Wuhan 430068;Huangshi Maternal and Child Health Hospital of Hubei Province,Wuhan 435003;Department of Nephrology,East Hospital,Wuhan University People′s Hospital,Wuhan 430060,China)

机构地区：[1]湖北工业大学工业发酵省部共建协同创新中心,湖北武汉430068 [2]湖北工业大学细胞调控与分子药物学科创新引智基地(“111基地”),湖北武汉430068 [3]湖北工业大学孤独症抑郁症诊断与干预研究所,湖北武汉430068 [4]湖北工业大学大健康与精准医药营养国际联合研究中心,湖北武汉430068 [5]湖北工业大学生物工程与食品学院,湖北武汉430068 [6]湖北省黄石市妇幼保健院,湖北黄石435003 [7]武汉大学人民医院东院肾内科,湖北武汉430060

出　　处：《生物技术》2024年第5期636-640,635,共6页Biotechnology

基　　金：国家自然科学基金项目(82061138005);湖北省教育厅创新团队项目(T2020009)。

摘　　要：[目的]为了寻找能够区分孤独症谱系障碍(ASD)、智力障碍(ID)两种疾病的潜在生物标志物。[方法]对招募的ASD、ID儿童进行血浆和血清样本采集,使用一氧化氮分析仪、高效液相色谱和紫外分光光度计检测亚硝酸盐、硝酸盐、丙二醛(MDA)和总抗氧化能力(T-AOC)4种氧化应激相关指标,并比较两组各指标差异。[结果]在血浆中,ASD儿童亚硝酸盐显著高于ID儿童亚硝酸盐,硝酸盐、MDA和T-AOC在两组间无显著差异。血清中亚硝酸盐、硝酸盐、MDA和T-AOC在ASD和ID儿童中均无显著性差异。硝酸盐和T-AOC在血浆和血清中呈显著正相关,亚硝酸盐和MDA在血清和血浆中无相关性。[结论]ASD、ID儿童中氧化应激水平存在差异,血浆亚硝酸盐水平可以作为区分两种疾病的潜在生物标志物。[Objective]To find potential biomarkers that can distinguish between autism spectrum disorder(ASD)and intellectual disability(ID).[Method]Plasma and serum samples were collected from the recruited children with ASD and ID.Nitrite,nitrate,malondialdehyde(MDA),and total antioxidant capacity(T-AOC)were detected by nitric oxide analyzer,high performance liquid chromatography,and ultraviolet spectrophotometer.The differences of oxidative stress indexes between ASD and ID were compared.[Result]In plasma,nitrite was significantly higher in ASD children than in ID children.Nitrate,MDA,and T-AOC were not significantly different between the two groups.Serum nitrite,nitrate,MDA,and T-AOC were not significantly different between ASD and ID children.There was a significant positive correlation between nitrate and T-AOC in plasma and serum,but no correlation between nitrite and MDA in serum and plasma.[Conclusion]There were differences in oxidative stress levels between children with ASD and ID,and plasma nitrite levels can be used as a potential biomarker to distinguish between the two disorders.

关 键 词：孤独症谱系障碍 智力障碍 氧化应激 一氧化氮 亚硝酸盐 硝酸盐 丙二醛 总抗氧化能力 

分 类 号：R749.94[医药卫生—神经病学与精神病学]