生菜中重要致病微生物污染与耐药性分析  

Analysis of contamination and resistance of important pathogenic microorganisms in lettuce

作　　者：宋学敏 蔡丹丹[1] 于喜喜 张璇 朱伟光[1] SONG Xue-Min;CAI Dan-Dan;YU Xi-Xi;ZHANG Xuan;ZHU Wei-Guang(Suqian Center for Disease Control and Prevention,Suqian 223800,China;School of Publicalth,Nanjing Medical University,Nanjing 211166,China)

机构地区：[1]宿迁市疾病预防控制中心,宿迁223800 [2]南京医科大学公共卫生学院,南京211166

出　　处：《食品安全质量检测学报》2024年第20期118-124,共7页Journal of Food Safety and Quality

基　　金：宿迁市科技局市级科技计划项目(Z2021067);2023—2025年度江苏省食品安全风险监测微生物重点实验室任务项目。

摘　　要：目的分析生菜中重要致病微生物污染情况及菌株的耐药性。方法在宿迁市种植、流通、餐饮等环节采集312份生菜,进行致病微生物检测和鉴定,并对分离到的菌株进行药敏测定。结果生菜中共检出了致病微生物38株;流通环节的检出率最高(21.64%),检出差异有统计学意义(χ^(2)=16.960,P<0.001);24株大肠埃希氏菌中,有13株对至少一类抗菌药物耐药,主要为氨苄西林(50.00%)和萘啶酸(50.00%),多重耐药率为50.00%(12/24);6株沙门氏菌对抗菌药物均产生不同程度的耐药,主要是四环素(100.00%)和米诺环素(100.00%),4株存在多重耐药。结论生菜中致病微生物检出率较高,检出的沙门氏菌和大肠埃希氏菌存在一定程度的多重耐药情况,应该持续开展监测。Objective To analyze the contamination of important pathogenic microorganisms and their resistance in lettuce.Methods The 312 lettuce samples were collected from planting,circulation,and catering in Suqian City.Pathogenic microorganisms were detected and identified,and the drug susceptibility of isolated strains was determined.Results A total of 38 diarrheagenic Escherichia coli strains of pathogenic bacteria were detected in lettuce.The detection rate of circulation was the highest(21.64%),and the difference was statistically significant(χ^(2)=16.960,P<0.001).Among 24 strains of Escherichia coli,13 strains were resistant to at least one class of antimicrobial agents,mainly ampicillin(50.00%)and nalidixic acid(50.00%),and the multiple resistance rate was 50.00%(12/24).Six strains of Salmonella differed in their drug susceptibility to antibiotics,mainly tetracycline(100.00%)and minocycline(100.00%),and 4 strains had multiple resistance.Conclusion The detection rate of pathogenic microorganisms in lettuce is high,and the Salmonella and diarrheagenic Escherichia coli detected have multidrug-resistant to a certain extent,which should be continuously monitored.

关 键 词：生菜 致病菌检测 抗生素耐药性 食源性微生物污染 

分 类 号：R155.5[医药卫生—营养与食品卫生学]